Functional Genomics: A Practical Approach: 235 - Brossura

 
9780199637744: Functional Genomics: A Practical Approach: 235
Brossura
ISBN 10: 0199637741 ISBN 13: 9780199637744
Casa editrice: OUP Oxford, 2000

Sinossi

With the complete genomes of many organisms now available, and the first draft of the human genome imminent, there is an increasing demand from researchers in a range of disciplines for techniques that will allow them to utilize these resources in their own research. This book provides a comprehensive treatment of the range of methods available for gene and protein expression profiling in a variety of systems. These include large-scale methods, such as cDNA microarrays, serial analysis of gene expression (SAGE) and proteomics, and also methods suitable for non-specialist laboratories, such as differential display and suppression subtraction hybridization. In all cases the chapters have been written by the developers of these methods or experienced users and include detailed protocols to facilitate the introduction of these methods to the readers' laboratories.

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Recensione

" ... chapters are well organized and the protocols are clearly highlighted ... a good starting point for anybody interested in a 'practical approach' to the topic of gene expression." Trends in Biochemical Sciences

Contenuti

  • Functional Genomics: approaches and methodologies
  • Construction and Screening of a Subtractive cDNA Library
  • Differential Display Analysis of Alterations in Gene Expression
  • Representational Difference Analysis of cDNA
  • Identification of Differentially Expressed Genes by Suppression Subtractive Hybridization (SSH)
  • Gene Expression Analysis by cDNA Microarrays
  • SADE: A Microassay for Serial Analysis of Gene Expression
  • Analysis of Gene Expression in Single Cells Using Three Prime End Amplification (TPEA) PCR
  • Analysis of Gene Expression by 2-D Gel Electrophoresis
  • Proteome Research: methods for protein characterization

Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.

Editore
OUP Oxford
Data di pubblicazione
2000
Lingua
Inglese
ISBN 10 
0199637741
ISBN 13 
9780199637744
Rilegatura
Copertina flessibile
Numero di pagine
272
Contatto del produttore
non disponibile
Persona responsabile
Penguin Random House Ireland Limited
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