Cytoskeleton: Signalling and Cell Regulation: A Practical Approach: 221 - Rilegato

 
9780199637829: Cytoskeleton: Signalling and Cell Regulation: A Practical Approach: 221

Sinossi

This book provides descriptions of experimental methods in research on the cytoskeleton and its relationships to signaling and cell regulation. Thus, it bridges two active and fertile areas of research. The focus is directed particularly towards methods which take advantage of recent advances in molecular biology, microscopy and immunological assays. A second emphasis is on methods for understanding dynamic changes in cells. A third emphasis is on the formation and turnover of macromolecular and supramolecular complexes, which are so important in driving cell regulation and the behaviour of cytoskeletal elements. A combination of practical advice and detailed protocols should make this book valuable for both novice and experienced workers in these burgeoning fields.

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Contenuti

  • Preface
  • 1: Carraway: Introduction: The cytoskeleton in the transduction of signal and regulation of cellular function
  • 2: Ampe and Vandekerckhove: Assaying binding and covalent modifications of cytoskeletal proteins
  • 3: Karpova and Cooper: Methods to study actin assembly and dynamics in yeast
  • 4: Carraway and Carraway: Signaling complexes: Association of signaling proteins with the cytoskeleton
  • 5: Ridley and Flinn: Ras-related GTPases and the cytoskeleton
  • 6: Fishkind et al: Applications for analysis of contractile functions in dividing and migrating tissue culture cells
  • 7: Zeile et al: Video microscopy: Protocols for examining the actin-based motility of Listeria, Shigella, Vaccinia, and Lanthanum-induced endosomes.
  • 8: Downing: Structural studies on tubulin and microtubules
  • 9: Inada et al: Regulation of Intermediate Filament Dynamics
  • 10: Bermano and Hesketh: The study of mRNA-cytoskeleton interactions and mRNA sorting in mammalian cells
  • 11: Goldmann et al: Cell shape control and mechanical signaling through the cytoskeleton
  • Appendix
  • Index

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