Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre- loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
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"The book can be warmly recommended as a valuable advisor for researchers in the fields of mouse genetics and cell biology" Cell Biology International
" ... a collection of state-of-the-art protocols" Cambridge Scientific Abstracts
Alexandra Joyner is at HHMi and Skirball Institute of Biomolecular Medicine, NYU Medical Centre.
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Descrizione libro Paperback. Condizione libro: New. Brand New! We ship daily Monday - Friday!. Codice libro della libreria 1EYCZC0005V6
Descrizione libro Condizione libro: Brand New. Book Condition: Brand New. Codice libro della libreria 97801996379281.0
Descrizione libro Oxford University Press, USA, 2000. Paperback. Condizione libro: New. 2. Codice libro della libreria DADAX019963792X
Descrizione libro Oxford University Press, USA, 2000. Paperback. Condizione libro: Brand New. 2nd edition. 293 pages. 9.25x6.25x0.75 inches. In Stock. Codice libro della libreria zk019963792X