Sinossi
Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.
Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.
Recensione
"... the book contains extremly useful practical advice for studying lymphocytes in the laboratory..."Parasitology Today
Contenuti
- Preface
- Plebanski: Preparation of lymphocytes and idenfication of lymphocyte subpopulations
- Biddulph and Gatter: Immunohistochemistry of lymphoid organs
- Meinl and Fickenscher:
- Bastin: T and B-cell hybridomas
- Mills: Murine T-cell culture
- Vyakarnam: Human CD4+ T-cell culture
- Rowland-Jones: Human Cytotoxic T-cell culture
- Carmichael: Limiting dilution analysis for quantification of antigen-specific T-cells
- Ogg: HLA -peptide tetrameric complexes
- Tan: Expansion of human T-cells for immunotheraphy
- Browning and Krausa: HLA typing
- Brown and Symons: Characterisation of lymphocyte surface markers
- Bangham and Furukawa: Apoptosis assays for lymphocytes
- Owen and McLoughlin: Thymic organ culture
- Index
Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.
