In Situ Hybridization in Electron Microscopy - Brossura

Morel, Gerard; Cavalier, Annie; Williams, Lynda

Brossura

ISBN 10: 0367455374 ISBN 13: 9780367455378

Casa editrice: CRC Press, 2020

Riassunto

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization of genes: ° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method) ° hybridization prior to embedding (pre-embedding) ° hybridization on ultrathin sections of frozen tissue (frozen tissue method). For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products. The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.

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Informazioni sull'autore

Morel, Gerard | Cavalier, Annie | Williams, Lynda

Dettagli dell'edizione

• Editore: CRC Press
• Data di pubblicazione: 2020
• Lingua: Inglese
• ISBN 10: 0367455374
• ISBN 13: 9780367455378
• Rilegatura: Copertina flessibile
• Numero edizione: 1
• Numero di pagine: 472
• Contatto del produttore: Taylor and Francis, GPSR Taylor and Francis; gpsr@taylorandfrancis.com; ; Taylor & Francis Verlag GmbH, Kaufingerstraße 24; München; 80331; Germania