This Second Edition incorporates the latest developments in the practical application of LC. Covers the basic of LC, the six LC methods and their applications, and various specialized areas. Provides material for in-depth comprehension of how HPLC is performed, what the necessary materials are, and possible applications. Offers thorough treatment of such areas as quantitative and qualitative analysis by HPLC, preparative scale separations, gradient elution and column-switching, sample pretreatment and reaction detectors, automated systems for high-volume testing and/or samples requiring pretreatment, troubleshooting and sample artifacts, and selection and development of LC method for a particular applications.
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The latest edition of the authoritative reference to HPLC
High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples.
This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as:
The basis of HPLC separation and the general effects of different experimental conditions
Equipment and detection
The column—the "heart" of the HPLC system
Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques
Computer simulation, qualitative and quantitative analysis, and method validation and quality control
The separation of large molecules, including both biological and synthetic polymers
Chiral separations, preparative separations, and sample preparation
Systematic development of HPLC separations—new to this edition
Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms
Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.About the Author:
Lloyd R. Snyder, PhD, is a Principal at LC Resources in Walnut Creek, California. He is the author or coauthor of several books including An Introduction to Separation Science, Introduction to Modern Liquid Chromatography, Second Edition, the bestselling Practical HPLC Method Development, Second Edition, and the comprehensive High-Performance Gradient Elution, all published by Wiley.
Joseph J. Kirkland, PhD, is Vice President of Research and Development for Advanced Materials Technology, Inc., and coauthor of Introduction to Modern Liquid Chromatography, Second Edition, Practical HPLC Method Development, Second Edition, and Modern Size-Exclusion Liquid Chromatography, Second Edition, all published by Wiley.
John W. Dolan, PhD, is a Principal at LC Resources. He is author of the popular "LC Troubleshooting" column in LCGC magazine and coauthor with Lloyd Snyder of Troubleshooting LC Systems and High-Performance Gradient Elution.
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Descrizione libro Wiley-Interscience, 1979. Hardcover. Condizione libro: New. book. Codice libro della libreria 0471038229
Descrizione libro Wiley-Interscience, 1979. Hardcover. Condizione libro: New. 2. Codice libro della libreria DADAX0471038229
Descrizione libro Wiley-Interscience, 1979. Hardcover. Condizione libro: New. Codice libro della libreria P110471038229
Descrizione libro Wiley-Interscience. Hardcover. Condizione libro: New. 0471038229 New Condition. Codice libro della libreria NEW6.3094182
Descrizione libro Condizione libro: Brand New. Book Condition: Brand New. Codice libro della libreria 97804710382211.0