This is a second generation genetic manipulation book. It goes beyond the simple cloning of a gene to describe and evaluate the concepts and approaches that are currently pushing back the frontiers of eukaryotic molecular biology and forming the basis of a technological revolution. The book is in three parts. Part 1 outlines the structure and function of the genome and describes basic techniques in molecular biology. In Part 2 selected examples are used to illustrate the major areas of molecular biology that are progressing via gene transfer technology. These range from fundamental aspects of gene and protein structure and function through to applications in biotechnology and medicine. Part 3 begins with a refresher chapter on recombinant DNA techniques and then goes on to describe what may be done with a cloned gene.
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