Autoradiography and Immunocytochemistry: Practical Methods in Electron Microscopy: v.6 - Brossura

Williams, M. A.

Brossura

ISBN 10: 0720406374 ISBN 13: 9780720406375

Casa editrice: North-Holland, 1980

Riassunto

Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining” with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labeled tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.

Dettagli dell'edizione

• Editore: North-Holland
• Data di pubblicazione: 1980
• Lingua: Inglese
• ISBN 10: 0720406374
• ISBN 13: 9780720406375
• Rilegatura: Copertina flessibile
• Numero di pagine: 218
• Contatto del produttore: non disponibile
• Persona responsabile: non disponibile