Learn to apply powerful molecular biology and genetics techniques to your microbial research with this easy-to-understand, practical guide. Microbial Genome Methods distills complex experimental procedures into fundamental techniques that can be readily adapted to your own research. In addition, this book provides background information specifically relevant to microbial research, as well as descriptions of expected results. The book is divided into two sections. Section one covers eukaryotic microbes, with emphasis on the yeast Saccharomyces cerevisiae. Section two covers prokaryotic microbes, focusing on Escherichia coli, and other important bacteria. These microbes were selected because they are widely studied and understood, but all information can be easily applied to the microbe you are investigating. Chapters are written by nationally and internationally recognized research scientists currently active in this field. Don't miss out on the tremendous boost these research techniques can give your work.
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Eukaryotic Microbes
Molecular Dissection of G Protein-Mediated Signal Transduction Using Random Mutagenesis in Dictyostelium, C.A. Parent and P.N. Devreotes
Cloning and Characterizing Developmentally Regulated Genes in Candida albicans, D.R. Soll, T. Srikantha, and S.R. Lockhart
Methods in the Analysis of Telomere Function in Saccharomyces cerevisiae, A.J. Lustig
Generation, Screening, and Analysis of LACZ Fusions in Yeast, N. Burns, P. Ross-MacDonald, G.S. Roeder, and M. Snyder
Development of a Genetic Selection for Transcription Factor Mutants Based on a Promoter Fusion to a G418 Resistance Gene in Saccharomyces cerevisiae, A.E. Pepper and M.J. Holland
Generation of Chimeric Proteins by in Vivo Homologous Gene Recombination in Yeast: Example of an Essential P-Type ATPase, A. de Kerchove d'Exaerde, P. Supply, and A. Goffeau
Comparative Gene Analysis and Dominant Negative Mutations in DNA Repair, G.T. Milne, J.W. Donovan, Y. Xiao, and D.T. Weaver
DNA Repair and Checkpoint Controls in Fission Yeast: A Practical Guide, A.M. Carr and J.M. Murray
Prokaryotic Microbes
Techniques for the Introduction of Specific DNA Lesions Downstream from Prokaryotic RNA Polymerase Promoters and Analysis of the Transcription Products, W. Zhou and P.W. Doetsch
Bacteria Identification by PCR-Based Techniques, G. Damiani, P. Amedeo, C. Bandi, R. Fani, D. Bellizzi, and V. Sgaramella
Live Time Quantification of Bacterial Interactions in Various Environments, C.I. Kado
Isolation of Complexes Containing an Escherichia coli Chaperone Protein, SecB, Bound to Newly Synthesized Polypeptides, O. Francetic and C.A. Kumamoto
Pathogenesis and Host Resistance to Infection: A Model System and an Analysis of Capsule Synthesis and Regulation by Bacillus anthracis, S.L. Welkos and R. Marrero
Genetic Manipulation of Bordetella, V. Scarlato, S. Ricci, R. Rappuoli, and M. Pizza
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