Since its development in the 1970s, computerized image analysis has witnessed rapid growth and enormous progress. Powerful hardware and robust algorithms have laid the groundwork for highly specialized, automatic image analysis, which has found applications in all fields of biology and medicine. Light microscopy, electron microscopy, and medical sampling have all benefited from this new technology. Image Analysis in Biology presents a comprehensive look at some of the latest advances in image analysis. The book introduces readers to image analysis with overviews of 3-D analysis, quantitation by laser scanning, confocal microscopy, and quantitative area determination image analysis. It then focuses on the applications of image analysis in microscopy, including contrast enhancement in video images, automatic detection, and recognition of live cells. Other topics presented include the analysis of subcellular structures in electron micrographs and protein analysis; the reconstruction of three-dimensional objects from serial section images; and techniques used for movement analysis of microorganisms, bacteria, and animals, which require fast routines to track the organisms in real time in a sequence of video frames. Microbiologists, cell biologists, botanists, photochemists, and other researchers involved in fields that utilize computerized image analysis will find a tremendous amount of useful information in this timely volume.
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INTRODUCTION. METHODS OF IMAGE ANALYSIS. Principles of Object Detection for an Automated Cell Tracking System (David Gauthier, Martin D. Levine, and Peter B. Noble). Quantitative Area Determination by Computer Image Analysis (Alan C. Bovik, Shanti J. Aggarwal, Nak H. Kim, and Kenneth R. Diller). Digital Filters in Image Analysis (Roberto Marangoni, Giuliano Colombetti, and Paolo Gualtieri). Single Photon Imaging (Tsuyoshi Hayakawa). Analysis of Two-Dimensional Electrophoresis Gels (Donat-P. Häder). MICROSCOPY AND ULTRAMICROSCOPY. Confocal Image Cytometry-Quantitative Analysis of Three-Dimensional Images Obtained by Confocal Scanning Microscopy (Jean Paul Rigaut, Santos Carvajal-Gonzalez, and Jany Vassy). Video-Microscopic Techniques to Study the Living Cytoplasm (Dieter G. Weiss and Günther Galfe). Image Processing and Analysis Relating to Structure and Function in Eukaryotic Flagella and Cilia (Michael E.J. Holwill and Peter Satir). Image Analysis of Stomatal Movements and Gas Exchange (Kenji Omasa and Judith G. Croxdale). Processing and Analysis of Electron Images of Biomolecules (George Harauz and Egbert J. Boekema). Three-Dimensional Reconstruction of Single Particles in Electron Microscopy (Michael Radermacher). Computer-Aided Three-Dimensional Reconstruction from Serial Section Images (Norio Baba). Automatic Grain Counting in Autoradiographs by Computerized Pattern Recognition (Nico A.M. Schellart). MOVEMENT ANALYSIS. Real Time Tracking of Microorganisms (Donat-P. Häder and Kurt Vogel). Automated Measurement of Movement Responses in Halobacteria (Tetsuo Takahashi). Simultaneous Tracking of Multiple Subjects (David B. Dusenbery). Image Analysis of Motile Flagellates (Paolo Gualtieri and Primo Coltelli). INDEX. c. 256 pp., 172 illus., 7x10
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