This illustrated volume surveys the correlated use of currently available methods of electron microscopic techniques, along with the goals and perspectives for future developments. The authors discuss an integrative approach of different EM preparation and analysis techniques that can allow for an analysis of dynamic cellular processes with high temporal and spatial resolution on the electron microscope level. This concise, yet thorough, work is a valuable reference for researchers in the field.
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Current Trends in the Electron Microscopic Analysis of Dynamic Processes in the Field of Cell and Molecular Biology (H. Plattner). Self-Assembly of Crystalline Bacterial Cell Surface Layers (S-Layers) (U.B. Sleytr and P. Messner). Gap Junctions: Correlated Electrophysiological Recordings and Ultrastructural Analysis by Fast Freezing and Freeze-Fracturing (D.F. Hülser, D. Paschke, and J. Greule). Analysis of Irregular Distributions and Dynamics of Membrane Components by Computer-Aided Image Recognition and Statistical Evaluation (S.W. Hui). Lipid Phase Transitions and Micro-Domains in Biomembranes and Their Possible Relevance for Biological Processes (A.J. Verkleij). Ultrastructural Analysis of Biological Membrane Fusion and a Tentative Correlation with Biochemical and Biophysical Aspects (G. Knoll and H. Plattner). Analysis of Dynamic Cell Processes by Rapid Freezing and Freeze Substitution (E. Morgenstern and L. Edelmann). Topography and Internalization of Cell-Surface Receptors as Analyzed by Affinity- and Immuno-Labeling Combined with Surface Replication and Ultrathin Sectioning Techniques (H. Robenek). Enzymatic Labeling of Cell-Surface Glycoproteins as an Autoradiographic Probe for Morphometric Analysis of Intracellular Membrane Traffic (L. Thilo and C. de Chastellier). Low-Temperature Embedding and Cryosectioning in the Immunocytochemical Study of Membrane Recycling (D. Brown). Pre-embedding Labeling Techniques Applicable to Intracellular Binding Sites (M. Pavelka and A. Ellinger). Organizational Principles of the Golgi Apparatus: Contributions from Postembedding Gold Labeling Techniques (J. Roth and D.J. Taatjes). Neoglycoproteins as Tools to Analyze Endogenous Lectins and Glycoconjugates (M. Monsigny, J. Hubert, A. Obrénovitch, and J. Schrével). Interaction between Cytoskeleton and Plasma Membrane in Virus Morphogenesis (W. Bohn). Scanning Electron Microscopy of Cell Surface Antigens Labeled with Colloidal Gold Markers (E. de Harven). Immunocytochemistry of Contractile and Cytoskeletal Proteins (J.V. Small). Analysis of Complex Three-Dimensional Structures Involved in Dynamic Processes by High Voltage Electron Microscopy and Low Voltage High Resolution Scanning Electron Microscopy (H. Ris and J.B. Pawley). X-Ray Microanalysis of Ion Changes during Fast Processes in Cells, as Exemplified by Trichocyst Exocytosis of Paramecium caudatum (M. Schmitz and K. Zierold). Correlated Electrostimulation and Electron Probe X-Ray Microanalysis of Heart Muscle Cells (M.F. Wendt-Gallitelli and G. Isenberg). Index.
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Da: Biblio Pursuit, Lenhartsville, PA, U.S.A.
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