Handbook of Cryo-Preparation Methods for Electron Microscopy

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9780849372278: Handbook of Cryo-Preparation Methods for Electron Microscopy

While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable.

The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations.

Contributions to this handbook –

Ö         Describe cryo-methods aimed at perfect preservation for fine structural analysis

Ö         Teach how to arrest physiological processes by cryo-fixation

Ö         Reveal the secrets for high-resolution snapshots of life by CEMOVIS

Ö         Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography

Ö         Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules

Ö         Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography

Ö         Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding

Ö         Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling

Ö         Include summary tables to compare and obtain appropriate criteria

Includes a wealth of immediate reference material for daily use

With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.

Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.

Contenuti:

Cryo-Fixation Methods. Macromolecular Assemblies. Cryo-ultramicrotomy. Low-Temperature Embedding. Freeze-Fracture and Metal Shadowing. Immunolabeling, Electron Tomography.

Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.

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ISBN 10: 0849372275 ISBN 13: 9780849372278
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CAVALIER, ANNIE; SPEHNER, DANIELE; HUMBEL, BRUNO M.
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Descrizione libro CRC Press, 2017. Hardback. Condizione libro: NEW. 9780849372278 This listing is a new book, a title currently in-print which we order directly and immediately from the publisher. Print on Demand title, produced to the highest standard, and there would be a delay in dispatch of around 10 working days. Codice libro della libreria HTANDREE0249548

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Cavalier, Annie [Editor]; Spehner, Daniele [Editor]; Humbel, Bruno M. [Editor];
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Descrizione libro CRC Press, 2008. Hardcover. Condizione libro: New. Codice libro della libreria INGM9780849372278

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Cavalier, Annie (Edited by)/ Spehner, Daniele (Edited by)/ Humbel, Bruno M. (Edited by)
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Descrizione libro CRC Pr I Llc, 2008. Hardcover. Condizione libro: Brand New. 1st edition. 704 pages. 10.28x7.17x1.34 inches. In Stock. Codice libro della libreria __0849372275

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Descrizione libro CRC Press, 2008. HRD. Condizione libro: New. New Book. Shipped from US within 10 to 14 business days. THIS BOOK IS PRINTED ON DEMAND. Established seller since 2000. Codice libro della libreria I1-9780849372278

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Cavalier, Annie (EDT)/ Spehner, Daniele (EDT)/ Humbel, Bruno M. (EDT)
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Descrizione libro Taylor and Francis, 2008. HRD. Condizione libro: New. New Book. Shipped from US within 10 to 14 business days. Established seller since 2000. Codice libro della libreria VT-9780849372278

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Descrizione libro Taylor Francis Inc, United States, 2008. Hardback. Condizione libro: New. 254 x 182 mm. Language: English . Brand New Book ***** Print on Demand *****.While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable. The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations. Contributions to this handbook - O Describe cryo-methods aimed at perfect preservation for fine structural analysis O Teach how to arrest physiological processes by cryo-fixation O Reveal the secrets for high-resolution snapshots of life by CEMOVIS O Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography O Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules O Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography O Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding O Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling O Include summary tables to compare and obtain appropriate criteria Includes a wealth of immediate reference material for daily use With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research. Codice libro della libreria APC9780849372278

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Editore: Taylor Francis Inc, United States (2008)
ISBN 10: 0849372275 ISBN 13: 9780849372278
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Descrizione libro Taylor Francis Inc, United States, 2008. Hardback. Condizione libro: New. 254 x 182 mm. Language: English . Brand New Book ***** Print on Demand *****. While new discoveries have led to much dramatic growth in cryo-electron microscopy, researchers will never be able to take full advantage if they lack access to the details that make these techniques understandable and applicable. The Handbook of Cryo-Preparation Methods for Electron Microscopy provides researchers with a complete reference that will show them how to equip their labs with the right materials and methods to take full advantage of the latest advances. This pioneering work brings together a group of internationally renowned researchers, some the very inventors of the methods they describe, to share their knowledge and recipes. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the latest theory, principles, and protocols supplemented by hundreds of illustrations. Contributions to this handbook - O Describe cryo-methods aimed at perfect preservation for fine structural analysis O Teach how to arrest physiological processes by cryo-fixation O Reveal the secrets for high-resolution snapshots of life by CEMOVIS O Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography O Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules O Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography O Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding O Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling O Include summary tables to compare and obtain appropriate criteria Includes a wealth of immediate reference material for daily use With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research. Codice libro della libreria APC9780849372278

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