Nucleic Acids: 2 - Rilegato

Rilegato

ISBN 10: 0896030644 ISBN 13: 9780896030640

Casa editrice: SPRINGER NATURE, 1984

Riassunto

Although many of the new techniques in molecular biology are relatively easy to master, it is often difficult for a researcher to obtain all the relevant information nec- sary for setting up and successfully applying a new te- nique.

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Contenuti

The Burton Assay for DNA.- DABA Fluorescence Assay for Submicrogram Amounts of DNA.- Preparation of “RNase-Free” DNase by Alkylation.- The Isolation of Satellite DNA by Density Gradient Centrifugation.- The Isolation of High Molecular Weight Eukaryotic DNA.- Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids.- Agarose Gel Electrophoresis of DNA.- Autoradiography of Gels Containing 32P.- Detection of Specific DNA Sequences―The Southern Transfer.- The Extraction and Isolation of DNA from Gels.- One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography.- Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions.- The Extraction of Total RNA by the Detergent and Phenol Method.- RNA Extraction by the Proteinase K Method.- RNA Extraction by the Guanidine Thiocyanate Procedure.- The Purification of Poly(A)-Containing RNA by Affinity Chromatography.- Messenger RNA Fractionation on Neutral Sucrose Gradients.- The Estimation of mRNA Content by Poly(U) Hybridization.- DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts.- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System.- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System.- Immunoprecipitation of in Vitro Translation Products with Protein A Bound to Sepharose.- In Vitro Continuation of RNA Synthesis Initiated in Vivo.- Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA.- Plasmid DNA Isolation by the Cleared Lysate Method.- Plasmid DNA Isolation (Sheared Lysate Method).- Small-Scale Plasmid DNA Preparation.- Preparation of Chromosomal DNA from E. coli.- Preparation and Assay of Phage Lambda.- Preparation of Phage Lambda DNA.- The Use of Restriction Endonucleases.- Ligation of DNA with T4 DNA Ligase.- The Use of Alkaline Phosphatase to Prevent Vector Regeneration.- Bacterial Transformation.- Bacterial Transformation (Kushner Method).- In Vitro Packaging of DNA.- Yeast Transformation.- Radiolabeling of DNA by Nick Translation.- Radiolabeling of DNA Using Polynucleotide Kinase.- Radiolabeling of DNA with 3? Terminal Transferase.- Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase.- Identification of Recombinant Plasmids by In Situ Colony Hybridization.- Identification of Recombinant Phages by Plaque Hybridization.- Plasmid Screening Using Single Colony Lysates.- Immunological Detection of Gene Expression in Recombinant Clones.- The Isolation of Minicells.- The Isolation of Maxicells.- The Identification of Gene Products in Minicells and Maxicells.- Molecular Cloning in Bacteriophage Lambda and in Cosmids.- DNA Transformation of Mammalian Cells.- Chemical Cleavage (Maxam and Gilbert) Method for DNA Sequence Determination.- Gel Electrophoretic Analysis of DNA Sequencing Products.- DNA Sequence Determination Using Dideoxy Analogs.

Product Description

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Dettagli dell'edizione

• Editore: SPRINGER NATURE
• Data di pubblicazione: 1984
• Lingua: Inglese
• ISBN 10: 0896030644
• ISBN 13: 9780896030640
• Rilegatura: Copertina rigida
• Numero di pagine: 375
• Redattore: Walker John M.
• Contatto del produttore: non disponibile
• Persona responsabile: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Germania