Articoli correlati a Enzymatic Analysis: A Practical Guide
Sinossi
Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity―all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.
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Recensione
...the authors are well recognized for their contributions to histochemistry...For individuals interested in...performing quantitative histochemical methods, this book would be a worthwhile investment.-Modern Pathology
Contenuti
General Principles: Pyridine Nucleotides. A Kinetics Primer for the Tissue Analyst. Constriction Pipets. Preparation of Tissues for Analysis. Specific Methods and Procedures: Enzymatic Cycling. A Collection of Metabolite Assays. A Collection of Enzyme Assays. Improvement, Modification, Adaptation, Troubleshooting, and Development of New Methods. Quantitative Histochemistry: Preparation of Tissues and Sections. Dissection and Histological Control. The Quartz Fiber Fishpole Balance. Histochemical Analysis. Appendix: References. Index.
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Enzymatic Analysis: A Practical Guide
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Humana Press, Totowa, New Jersey, 1993
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ISBN 13: 9780896032385
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Hardcover. Condizione: Near Fine - New condition. Condizione sovraccoperta: No dust jacket. NOT a library discard (illustratore). Second printing. Totowa, New Jersey: Humana Press, 1993. NOT print on demand. We have only this one copy, but it is available now and ready to ship today from Henderson, Nevada. The cover is in NEAR FINE condition, with only a little minor shelfwear. NEW and unread inside. NO owner's name or bookplate. Bound in the original brown laminated boards, lettered in gold. From the publisher: "Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivityall likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.". Second printing. Hardcover. Near Fine - New condition/No dust jacket. Illus. by NOT a library discard. 8vo. x, 406pp. Great Packaging, Fast Shipping. Codice articolo 031156
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Buch. Condizione: Neu. Neuware - Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity-all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step. Codice articolo 9780896032385
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