Articoli correlati a Chromosome Analysis Protocols: 29
Sinossi
Clear, step-by-step instructions provide you with all the techniques used in both classical and molecular cytogenetics. The emphasis is on the use of human material, but the techniques are designed to be readily applicable to other animal chromosomes. Chapters feature methods used in the preparation of chromosomes, in analyzing chromosomes directly at the light-microscope level, and in molecular genetics techniques.
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Recensione
...a very worthwhile book...invaluable for those trying to venture into the area of molecular cytogenetics...Provides a ready source of both old and new cytogenetic methods and contains a wealth of troubleshooting tips from experienced workers in the field.-Modern Pathology
Contenuti
Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution. Chromosome Preparation from Hematological Malignancies. Meiotic Chromosome Preparation. Preparation of Chromosomes for Scanning Electron Microscopy. Immortalized Cell Lines: Chromosome Preparation and Banding. Chromosome Banding and Identification: Absorption Staining. Chromosome Banding and Identification: Fluorescence. Chromosome Banding: Stain Combinations for Specific Regions. Inhibition of Chromosome Condensation. Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity. Automated Karyotype Analysis. Bivariate Chromosome Analysis Using a Commercial Flow Cytometer. Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping. Electrophoretic Karyotype Analysis: Pulse Field Gel Electrophoresis. Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations. Immunocytochemical Techniques Applied to Meiotic Chromosome Preparations. Immortalized Cell Lines: Their Creation and Use in Gene Mapping. Oligonucleotide-Primed In Situ DNA Synthesis: An Alternative to In Situ Hybridization for Gene Mapping and the Investigation of Genome Organization. Electron Microscopic Localization of In Situ Hybrids. Protocols for Chromosome Mediated Gene Transfer: Selection Strategies, Transgenome Analysis, Enrichment Cloning, and Mapping. Construction of Chromosome Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines. Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping: Amplification of Internal and Terminal Fragments by PCR, and Generation of Fingerprints. Chromosome Dissection and Cloning. Molecular Analysis of Chromosome Aberrations in Hematological Malignancies: Southern Hybridization. Molecular Analysis of Chromosome Aberrations: In Situ Hybridization. Alu- and L1-Primed PCR-Generated Probes for Non-Isotopic In Situ Hybridization. Chromosome Substructure Investigation:Telomeres. Index.
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Chromosome Analysis Protocols (Methods in Molecular Biology, Band 29)
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Humana press Totowa, 1994
ISBN 10: 0896032434
ISBN 13: 9780896032439
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Spiralbuch. Condizione: Gut. XI; 508 Seiten Das hier angebotene Buch stammt aus einer teilaufgelösten wissenschaftlichen Bibliothek und trägt die entsprechenden Kennzeichnungen (Rückenschild, Instituts-Stempel.); leichte altersbedingte Anbräunung des Papiers; der Buchzustand ist ansonsten ordentlich und dem Alter entsprechend gut. In ENGLISCHER Sprache. Sprache: Englisch Gewicht in Gramm: 830. Codice articolo 1698538
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Chromosome Analysis Protocols
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Condizione: Sehr gut. Zustand: Sehr gut | Seiten: 524 | Sprache: Englisch | Produktart: Bücher | Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reports¿as a result of pressure on space from editors, and the response to that pressure by authors¿contain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en countered in handling any biological system. This was the require ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series. Codice articolo 3645243/202
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Chromosome Analysis Protocols
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Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reports-as a result of pressure on space from editors, and the response to that pressure by authors-contain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en countered in handling any biological system. This was the require ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series. 524 pp. Englisch. Codice articolo 9780896032439
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Chromosome Analysis Protocols (Methods in Molecular Biology, 29)
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Chromosome Analysis Protocols
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Humana Press, 1994
ISBN 10: 0896032434
ISBN 13: 9780896032439
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Chromosome Analysis Protocols (Methods in Molecular Biology)
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Chromosome Analysis Protocols
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Taschenbuch. Condizione: Neu. Chromosome Analysis Protocols | John R. Gosden | Taschenbuch | xi | Englisch | 1994 | Humana Press | EAN 9780896032439 | Verantwortliche Person für die EU: Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. Codice articolo 101943172
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Chromosome Analysis Protocols
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Condizione: New. This book provides step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. Editor(s): Gosden, John R. Series: Methods in Molecular Biology. Num Pages: 509 pages, biography. BIC Classification: PSB; PSD; PSF. Category: (P) Professional & Vocational. Dimension: 156 x 234 x 26. Weight in Grams: 727. . 1994. Paperback. . . . . Codice articolo V9780896032439
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Condizione: New. pp. 508 1st Edition. Codice articolo 26285933
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Chromosome Analysis Protocols
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Humana Press, Humana Press Feb 1994, 1994
ISBN 10: 0896032434
ISBN 13: 9780896032439
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Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reports¿as a result of pressure on space from editors, and the response to that pressure by authors¿contain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en countered in handling any biological system. This was the require ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 524 pp. Englisch. Codice articolo 9780896032439
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