Sinossi
Patch-Clamp Applications and Protocols presents the broadest coverage of techniques for measuring and recording directly activity within a cell. The first part of the book offers modern developments associated with the technology of path-clamp electrodes, of cell-free ion-channel recording, and of the whole-cell patch clamp technique. Chapters on such recent offspring of the technique as the concentration clamp technique, the pressure clap method, and the perfusion of patch clamp electrodes are contributed by authors intimately involved with their development. Also covered is the loose patch-clamp technique, single-channel recording, the perforated patch-clamp technique, molecular biological aspects of the technique, and the use of the technique in brain slices. Every scientist working with path-clamp methods will find this protocols manual invaluable to their continued use and development of this powerful technique.
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Recensione
...valuable...because much of the book is written with a view to helping with the design of experiments and improving the quality of patch-clamp recordings...a useful addition and update to the technical literature on the patch-clamp technique. -Trends in Pharmacological Sciences
Contenuti
Technology of Patch-Clamp Electrodes. Whole-Cell Patch-Clamp Recordings. Pressure/Patch-Clamp Methods. Cell-Free Ion-Channel Recording. Perfusion of Patch Pipets. Concentration Clamp Techniques. Perforated Patch-Clamp Techniques. The Loose Patch Voltage Clamp Technique. Patch-Clamp Recording and RT-PCR on Single Cells. Patch-Clamp Technique in Brain Slices. Xenopus Oocyte Microinjection and Ion-Channel Expression. Index.
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Patch-Clamp Applications and Protocols: 26 (Neuromethods)
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Patch-Clamp Applications and Protocols: 26 (Neuromethods)
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gebundene Ausgabe. Condizione: Gut. 316 Seiten Das hier angebotene Buch stammt aus einer teilaufgelösten wissenschaftlichen Bibliothek und trägt die entsprechenden Kennzeichnungen (Rückenschild, Instituts-Stempel.); leichte altersbedingte Anbräunung des Papiers; der Buchzustand ist ansonsten ordentlich und dem Alter entsprechend gut. In ENGLISCHER Sprache. Sprache: Englisch Gewicht in Gramm: 740. Codice articolo 1670862
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Patch-Clamp Applications and Protocols - Neuromethods 26
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Patch-Clamp Applications and Protocols
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the membranbe of sk- etal muscles, thereby decreasing background membrane noise. In order to reduce the dominant source of background noise-the leakage shunt under the pipet rim between m- the muscle membrane had to be treated brane and gla- enzymatically. Despite these early limitations, a new te- nique was born -the patch-clamp technique. The final bre- through came in 1981 when the same authors, in collaboration with 0. P. Hamill, A. Marty, and F. J. Sigworth, developed the gigaohm seal. Not only did this improve the quality of recordings, it was now possible to gently pull the membrane patch with the attached pipet off the cell and study its trapped ion channels in isolation. Another offshoot of the gigaohm seal technique was the whole-cell patch-clamp technique, in which the patch is ruptured without breaking the seal. This technique is really a sophisticated voltage-clamp technique and also allows for the altering of cytoplasmic constituents if the experimenter so wishes. The first part of Patch-Clamp Applications and Protocols presents modern developments associated with the techn- ogy of patch-clamp electrodes, of cell-free ion channel reco- ing, and of the whole-cell patch-clamp technique. 336 pp. Englisch. Codice articolo 9780896033115
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Gebunden. Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. E. Neher and B. Sakman were the first to monitor the opening and closing of single ion channels and membranes by conductance measurements. In 1976, they used firepolished micropipets with a tip diameter of 3-5 pm to record currents from a small patch of the. Codice articolo 5982019
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