Protein Purification Protocols: v. 59 - Brossura

Brossura

ISBN 10: 0896033368 ISBN 13: 9780896033368

Casa editrice: Humana Pr Inc, 1996

Riassunto

A collection of recipes for protein fractionation and purification in a variety of experimental circumstances, offering step-by-step instructions on selecting a source, obtaining a usable initial extract, chemical and molecular purification, and drying and storing the purified protein. Includes material on special situations such as the use of subcellular fractionation and the use of electrophoresis for micropurification. For novice and experienced researchers. Plastic comb binding. Annotation c. by Book News, Inc., Portland, Or.

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Recensione

...includes some very useful contributions.- Angewandte Chemie

This book is recommended for protein chemists, whether beginner or professional. There is something in this book for everyone. It is an excellent working book as well as an excellent reference.-INFORM-American Oil Chemists Society

Doonan's handy compendium...holds its own against Methods in Enzymology [and] Current protocols in Protein Science... will be valuable to keep around the laboratory for most graduate students in biochemistry, and indeed for more senior practitioners of biotechnology as well. -Biopharm

Overall, a well presented utilitarian bench manual and a worthwhile purchase for novice and established practitioner alike-Society for General Microbiology Quarterly

Contenuti

General Strategies, Shawn Doonan. Preparation of Extracts from Animal Tissues, Shawn Doonan. Protein Extraction from Plant Tissues, Peter R. Shewry and Roger J. Fido. Extraction of Recombinant Protein from Bacteria, D. Margaret Worrall. Protein Extraction from Fungi, Paul Bridge. Subcellular Fractionation of Animal Tissues, Norma M. Ryan. Subcellular Fractionation of Plant Tissues: Isolation of Chloroplasts and Mitochondria from Leaves, Alyson K. Tobin. The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds, William S. Pierpoint. Avoidance of Proteolysis in Extracts, Robert J. Beynon and Simon Oliver. Concentration of Extracts, Shawn Doonan. Making and Changing Buffers, Shawn Doonan. Purification and Concentration by Ultrafiltration, Paul Schratter. Bulk Purification by Fractional Precipitation, Shawn Doonan. Ion-Exchange Chromatography, David Sheehan and Richard FitzGerald. Hydrophobic Interaction Chromatography, Paul O'Farrell. Affinity Chromatography, Paul Cutler. Dye-Ligand Affinity Chromatography, D. Margaret Worrall. Lectin Affinity Chromatography, Iris West and Owen Goldring. Immunoaffinity Chromatography, George W. Jack and David J. Beer. Immobilized Metal Ion Affinity Chromatography, Tai-Tung Yip and T. William Hutchens. Chromatography on Hydroxyapatite, Shawn Doonan. Affinity Precipitation Methods, Jane A. Irwin and Keith F. Tipton. Isoelectric Focusing, Reiner Westermeier. Chromatofocusing, Timothy J. Mantle and Patricia Noone. Size-Exclusion Chromatography, Paul Cutler. Fast Protein Liquid Chromatography (FPLC) Methods, David Sheehan. Reversed-Phase Chromatography of Proteins, Bill Neville. Extraction of Membrane Proteins, Kay Ohlendieck. Removal of Detergent from Protein Fractions, Kay Ohlendieck. Purification of Membrane Proteins, Kay Ohlendieck. Lyophilization of Proteins, Ciarán Ó Fágáin. Storage of Pure Proteins, Ciarán Ó Fágáin. Electroelution of Proteins from Polyacrylamide Gels, Michael J. Dunn. Electroblotting of Proteins from Polyacrylamide Gels, Michael J. Dunn. High-Performance Electrophoresis Chromatography, Serge Desnoyers, Sylvie Bourassa, and Guy G. Poirier. Practical Column Chromatography, Shawn Doonan. Index.

Dettagli dell'edizione

• Editore: Humana Pr Inc
• Data di pubblicazione: 1996
• Lingua: Inglese
• ISBN 10: 0896033368
• ISBN 13: 9780896033368
• Rilegatura: Copertina flessibile
• Numero di pagine: 405
• Redattore: Doonan Shawn
• Contatto del produttore: non disponibile
• Persona responsabile: non disponibile