Neurotransmitter Methods: 72 - Rilegato

Rilegato

ISBN 10: 0896033945 ISBN 13: 9780896033948

Casa editrice: Springer-Verlag GmbH, 1997

Riassunto

This outstanding and comprehensive collection of user-friendly protocols is essential for neuroscientists who wish to localize, identify, purify, and/or quantify neurotransmitters. The easily reproducible methods presented cover a broad spectrum of approaches involving cell and organ culture, histochemistry, immunochemistry, in situ hybridization, cell fractionation, liquid chromatography, mass spectrometry, in vivo microdialysis, and voltammetry. These approaches are optimized for use by neuroscientists, are often complementary, and can be employed in tandem with powerful effect.

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Contenuti

Preparation of Brain Slices, Ting Wang and Ira S. Kass. Preparation of Organotypic Hippocampal Slice Cultures Using the Membrane Filter Method, Peter J. Bergold and Patrizia Casaccia-Bonnefil. The Preparation and Use of Brain Slices and Dissociated Neurons for Patch-Clamp Studies of Neurotransmitter Action, Phillip M. Larkman and Hrvoje He´cimovi´c. Synaptosomes: A Model System to Study Release of Multiple Classes of Neurotransmitters, Alexandra I. M. Breukel, Elly Besselsen, and Wim E. J. M. Ghijsen. Synaptoneurosomes: A Preparation for Studying Subhippocampal GABAA Receptor Activity, Miriam N. G. Titulaer and Wim E. J. M. Ghijsen. Preparation of Synaptosomal Plasma Membranes by Subcellular Fractionation, Jacques J. H. Hens. Detection of Neuropeptides by Immunocytochemistry, Dick R. Nässel and Peter Ekström. Immunocytochemical Detection of Amino Acid Neurotransmitters in Paraformaldehyde-Fixed Tissues, David V. Pow. cGMP Immunocytochemistry, Jan de Vente and Harry W. M. Steinbusch. Microwave Antigen Retrieval in Formaldehyde-Fixed Human Brain Tissue, Suzanna J. Newman and Stephen M. Gentleman. Localization of Nitric Oxide Synthase Using NADPH Diaphorase Histochemistry, Maurice R. Elphick. In Situ Hybridization to Determine the Expression of Peptide Neurotransmitters, Niovi Santama. Quantitative In Situ Hybridization Histochemistry, Andrew Levy. Microbore Liquid Chromatography Analysis of Monoamine Transmitters, Sophie Sarre, Katrien Thorré, Ilse Smolders, and Yvette Michotte. Microbore Liquid Chromatography Analysis of Amino Acid Transmitters, Ilse Smolders, Sophie Sarre, Guy Ebinger, and Yvette Michotte. HPLC Methods to Isolate Peptide Neurotransmitters, G. Mark Holman and Timothy K. Hayes. Neuropeptide Expression Patterns as Determined by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry, Ka Wan Li and Wijnand P. M. Geraerts. GC/MS Determination of Biogenic Amines in Identified Neurons, David G. Watson, Richard A. Baines, John M. Midgely, and Jonathan P. Bacon. Monitoring Amino Acid Neurotransmitter Release in the Brain by In Vivo Microdialysis, Mark Zuiderwijk and Wim E. J. M. Ghijsen. In Vivo Detection of Neurotransmitters with Fast Cyclic Voltammetry, Julian Millar. Index.

Product Description

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Dettagli dell'edizione

• Editore: Springer-Verlag GmbH
• Data di pubblicazione: 1997
• Lingua: Inglese
• ISBN 10: 0896033945
• ISBN 13: 9780896033948
• Rilegatura: Copertina rigida
• Numero di pagine: 432
• Redattore: Rayne Richard C.
• Contatto del produttore: GPSR Kontakt; ProductSafety@springernature.com; ; Tiergartenstr. 17; Heidelberg; 69121; Germania