Prins and in Situ Pcr Protocols: 71 - Brossura

Brossura

ISBN 10: 0896033953 ISBN 13: 9780896033955

Casa editrice: Humana Pr Inc, 1996

Riassunto

Explains to molecular biologists the step-by-step procedures for using oligonucleotide primers to detect and amplify or extend complimentary sequences in situ , thus allowing the rapid identification and characterization of chromosomal DNA sequences, viral genomes, and rare messenger RNA in cells with a sensitivity and specificity hithertofore unmatched. Includes five color plates. The plastic claw binding lies flat for laboratory use. Annotation c. by Book News, Inc., Portland, Or.

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Recensione

...[a] splendid little book which covers the field quite well...Chapetr 16, which is a general review of in situ PCR written by Aidan Long and Paul Komminoth...is by far the best review of the approach...yet seen and should be mandatory reading for anyone intending to try in situ PCR. -FEBS Letters

"...useful to all those increasingly applying such techniques to in situ analysis...this book contains clear practical instructions that are easy to follow, and is well illustrated. It is essential for any laboratory contemplating exploiting this powerful technique."-SGM Quarterly

Contenuti

Oligonucleotide PRINS DNA Synthesis John R. Gosden and Diane Lawson. Chromosome-Specific PRINS Jean-Paul Charlieu and Franck Pellestor. Bright-Field Microscopic Detection of Oligonucleotide PRINS-Labeled DNA in Chromosome Preparations Ernst J. M. Speel, Daine Lawson, Frans C. S. Ramaekers, John R. Gosden, and Anton H. N. Hopman. Analysis of Sperm Aneuploidy by PRINS Franck Pellestor and Jean-Paul Charlieu. PRINS DNA Synthesis on Frozen Tissue Sections Ernst J. M. Speel, Diane Lawson, Frans C. S. Ramaekers, John R. Gosden, and Anton H. N. Hopman. Multiple Sequential Oligonucleotide Primed In Situ DNA Synthesis (MULTI-PRINS) John R. Gosden and Diane Lawson. Oligonucleotide PRINS DNA Synthesis on Extended Chromatin Preparations Yoshiro Shibasaki and John R. Gosden. Combined Immunocytochemistry and PRINS DNA Synthesis for Simultaneous Detection of Phenotypic and Genomic Parameters in Cells Ernst J. M. Speel, Diane Lawson, Frans C. S. Ramaekers, John R. Gosden, and Anton H. N. Hopman. Chromosomal PRINS DNA Labeling Combined with Indirect Immunocytochemistry Arthur R. Mitchell. Direct In Situ Single-Copy (DISC)-PCR Deryl Troyer, Hongyan Xie, and Jie Hu. In Situ PCR to Cells and to Wax Sections: An Alternative to In Situ Hybridization for the Detection of Low Copies of Human Papillomavirus and Cytomegalovirus DNA Christiane Mougin, Jean Marc Didier, Dominique Bettinger, Laurence Madoz, Sylviane Coumes-Marquet, and Michel Lab Reverse Transcriptase PCR In Situ on Cryopreserved Tissue Sections Fred J. Hendler, Alice Shum-Siu, Briana W. Heniford, Mohammed El Mekki Ahmed, and V. J. Patel Flow Cytometry Analysis of an In Situ PCR for the Detection of Human Immunodeficiency Virus Type-1 (HIV-1) Proviral DNA David E. Gibellini, Maria Carla Re, Giuliano Furlini, and Michele La Placa In Situ PCR Amplification of Intracellular mRNA Raymond H. Chen and Susan V. Fuggle. Localized In Situ Amplification (LISA): PCR Amplification of Nucleic Acid Sequences in Tissue Sections Gregory J. Tsongalis. In Situ PCR: An Overview Aidan A. Long and Paul Komminoth. Index.

Dettagli dell'edizione

• Editore: Humana Pr Inc
• Data di pubblicazione: 1996
• Lingua: Inglese
• ISBN 10: 0896033953
• ISBN 13: 9780896033955
• Rilegatura: Copertina flessibile
• Numero di pagine: 165
• Redattore: Gosden John R.
• Contatto del produttore: non disponibile
• Persona responsabile: non disponibile