Pichia Protocols - Rilegato

Rilegato

ISBN 10: 0896034216 ISBN 13: 9780896034211

Casa editrice: Humana Pr Inc, 1998

Riassunto

David Higgins and James Cregg compile for the first time an all-inclusive collection of key experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods - developed by world-class researchers in their own laboratories - contain detailed descriptions of available expression strains, as well as vectors and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Its detailed instructions will permit any researcher in molecular biology to quickly construct an expression strain that will produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.

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Recensione:

"In this valuable Methods in Molecular Biology volume, the editors have assembled an international cast of Pichia experts who provide detailed protocols, advice, and theory on recombinant expression in this methylotrophic yeast. . .a focused writing style that is enjoyable to read. Molecular geneticists are afforded the essential information to achieve success in vector construction, transformation and screening."-The Quarterly Review of Biology

Contenuti:

Introduction to Pichia pastoris, David R. Higgins and James M. Cregg. Classical Genetic Manipulation, James M. Cregg, Shigang Shen, Monique Johnson, and Hans R. Waterham. Transformation, James M. Cregg and Kimberly A. Russell. Small Vectors for Expression Based on Dominant Drug Resistance with Direct Multicopy Selection, David R. Higgins, Katie Busser, John Comiskey, Peter S. Whittier, Thomas J. Purcell, and James P. Hoeffler. The Generation of Multicopy Recombinant Strains, Mike Romanos, Carol Scorer, Koti Sreekrishna, and Jeff Clare. Isolation of Nucleic Acids David J. Miles, Katie Busser, Christine Stalder, and David R. Higgins. Generation of Protease-Deficient Strains and Their Use in Heterologous Protein Expression, Martin A. G. Gleeson, Christopher E. White, David P. Meininger, and Elizabeth A. Komives. Glycosylation Profiling of Heterologous Proteins, José Cremata, Raquel Montesino, Omar Quintero, and Rossana García.High Cell-Density Fermentation, Jayne Stratton, Vijay Chiruvolu, and Michael Meagher. Use of Pichia pastoris as a Model Eukaryotic System: Peroxisome Biogenesis, Klaas Nico Faber, Ype Elgersma, John A. Heyman, Antonius Koller, Georg H. Lüers, William M. Nuttley, Stanley R. Terlecky, Thibaut J. Wenzel, and Suresh Subramani. Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I), Russell A. Brierley. Secretion of scFv Antibody Fragments, Hermann Gram, Rita Schmitz, and Rüdiger Ridder. Expression of Tetanus Toxin Fragment C, Jeff Clare, Koti Sreekrishna, and Mike Romanos. Expression of EGF and HIV Envelope Glycoprotein, Jeff Clare, Carol Scorer, Rich Buckholz, and Mike Romanos. Expression of an Integral Membrane Protein, the 5HT5A Receptor, H. Markus Weiß, Winfried Haase, and Helmut Reiländer. Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections, Winfried Haase, H. Markus Weiß, and Helmut Reiländer. Appendix: Foreign Proteins Expressed in P. pastoris.Index.

Su questa edizione

• Editore: Humana Pr Inc
• Data di pubblicazione: 1998
• ISBN 10: 0896034216
• ISBN 13: 9780896034211
• Rilegatura: Copertina rigida
• Numero edizione: 1
• Numero di pagine: 270
• Redattore: Higgins David R., Cregg James M.