Aqueous Two-Phase Systems: Methods and Protocols: 11 - Rilegato

Hatt-Kaul, Rajni

Rilegato

ISBN 10: 0896035417 ISBN 13: 9780896035416

Casa editrice: Humana Press Inc., 2000

Riassunto

Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins - including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

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Dalla quarta di copertina

In Aqueous Two-Phase Systems: Methods and Protocols, Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications, all presented in set-by-step detail to ensure easy reproducibility and robust results. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Techniques for in situ product recovery during biocatalytic processes and for polymer-polymer systems in organic solvents are also presented.
Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Dettagli dell'edizione

• Editore: Humana Press Inc.
• Data di pubblicazione: 2000
• Lingua: Inglese
• ISBN 10: 0896035417
• ISBN 13: 9780896035416
• Rilegatura: Copertina rigida
• Numero di pagine: 454
• Contatto del produttore: non disponibile
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