Bacterial Toxins: Methods and Protocols: 145 - Rilegato

Rilegato

ISBN 10: 0896036049 ISBN 13: 9780896036048

Casa editrice: Humana Press Inc., 2000

Riassunto

Experienced researchers describe in step-by-step detail powerful methods for the investigation of bacterial exo- and endotoxins. These state-of-the-art techniques range from purification and detection methods to methods of conformational analysis, and include the use of phage antibody libraries and methods of structural and functional analyses of endotoxins. Each protocol is described by a scientist who has regularly used the method and optimized it to a high level of performance. Extensive notes deal with the difficulties that may arise when using the method and with the method's limitations and possible modification for other purposes. Cutting-edge and geared to producing successful experimental results, Bacterial Toxins: Methods and Protocols provides investigators with a first-rate collection of readily reproducible methods designed to help today's bacterial toxin investigators - both novice and expert - utilize a wide array of powerful research tools.

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Recensione

"The editor has put together an outstanding text on the current protocols used in the study of protein toxins. The caliber of the contributors is outstanding and represents an international perspective. While the editor does not present the text as an exhaustive discussion of microbial toxins, there is a focus on those areas where there is current and active research such as toxin-receptor kinetics, the use of fluorescence resonance energy transfer to detect structural changes in protein toxins, and CE-ESMS of short-chain lipopolysaccharides in Haemophilus influenzae. The organization and content level of this manual makes it a highly desirable addition to either a personal or a departmental library. "-Doody's Health Science Book Review Journal

Contenuti

Bacterial Protein Toxins: An Overview, Joseph E. Alouf. Purification of Clostridium botulinum Type A Neurotoxin, Carl J. Malizio, Michael C. Goodnough, and Eric A. Johnson. Shiga Toxins, David W. K. Acheson, Anne V. Kane, and Gerald T. Keusch. Isolation and Detection of Microcystins and Nodularins, Cyanobacterial Peptide Hepatotoxins, Jussi Meriluoto, Linda Lawton, and Ken-ichi Harada. Genetic Construction, Expression, and Characterization of Diphtheria Toxin-Based Growth Factor Fusion Proteins, Johanna C. vanderSpek and John R. Murphy. Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins, Jean-Louis Schwartz and Luke Masson. Use of Fourier-Transformed Infrared Spectroscopy for Secondary Structure Determination of Staphylococcal Pore-Forming Toxins, Gianfranco Menestrina. The Use of Fluorescence Resonance Energy Transfer to Detect Conformational Changes in Protein Toxins, William D. Picking. Site-Directed Spin Labeling of Proteins: Applications to Diphtheria Toxin, Kyoung Joon Oh, Christian Altenbach, R. John Collier, and Wayne L. Hubbell. Characterization of Molecular Properties of Pore-Forming Toxins with Planar Lipid Bilayers, Mauro Dalla Serra and Gianfranco Menestrina. Determination of Affinity and Kinetic Rate Constants Using Surface Plasmon Resonance, Luke Masson, Alberto Mazza, and Gregory De Crescenzo. ADP-Ribosylation of a-Gi Proteins by Pertussis Toxin: Positional Dissection of Acceptor Sites Using Membrane Anchored Peptides, Lars von Olleschik-Elbheim, Ali el Bayâ, and M. Alexander Schmidt. Phage Libraries for Generation of Anti-Botulinum scFv Antibodies, Peter Amersdorfer and James D. Marks. T-Cell Cytotoxicity Assays for Studying the Functional Interaction Between the Superantigen Staphylococcal Enterotoxin A and T-Cell Receptors, Alexander Rosendahl, Karin Kristensson, Kristian Riesbeck, and Mikael Dohlsten. In Vitro PhysiologicalStudies on Clostridial Neurotoxins: Biological Models and Procedures for Extracellularand Intracellular Application of Toxins, Bernard Poulain, Marie-France Bader, and Jordi Molgó. The Biology of Endotoxin, Volker T. El-Samalouti, Lutz Hamann, Hans-Dieter Flad, and Artur J. Ulmer. Matrix-Assisted Laser Desorption/ Ionization Time-of-Flight Mass Spectrometry of Lipopolysaccharides, Buko Lindner. Applications of Combined Capillary Electrophoresis-Electrospray Mass Spectrometry in the Characterization of Short-Chain Lipopolysaccharides: Haemophilus influenzae, Pierre Thibault and James C. Richards. Deacylation of Lipopolysaccharides and Isolation of Oligosaccharide Phosphates, Otto Holst. Electrophysiological Measurements on Reconstituted Outer Membranes, Andre Wiese and Ulrich Seydel. Index.

Dettagli dell'edizione

• Editore: Humana Press Inc.
• Data di pubblicazione: 2000
• Lingua: Inglese
• ISBN 10: 0896036049
• ISBN 13: 9780896036048
• Rilegatura: Copertina rigida
• Numero di pagine: 390
• Redattore: Holst Otto
• Contatto del produttore: non disponibile
• Persona responsabile: non disponibile