Pcr Mutation Detection Protocols: Methods in Molecular Biology: v. 187 - Brossura

Brossura

ISBN 10: 0896036170 ISBN 13: 9780896036178

Casa editrice: Humana Pr Inc, 2002

Riassunto

1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

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Recensione:

"The book provides access to the highly productive and readily reproducible PCR-based mutation detection methods, that are behind many of today's major scientific and medical advances." - Clinical Laboratory International

Contenuti:

Agarose and Polyacrylamide Gel Electrophoresis Andrea M. Guilliatt Internal Labeling of DNA Probes Ralph Rapley and Bimal D. M. Theophilus End-Labeling of DNA Probes Adrian J. Harwood Southern Blotting of Agarose Gels by Capillary Transfer Ralph Rapley and Ian J. Williams Restriction Fragment Length Polymorphism Mohammad S. Enayat PCR: Principles, Procedures, and Parameters Nicola Louise Jones Allele-Specific Oligonucleotide PCR Elaine K. Green Long-Range PCR, Peter A. Davies and George Gray Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing Anu Wartiovaara and Ann-Christine Syvänen Cycle Sequencing of PCR Products G. K. Surdhar Fluorescent In Situ Hybridization Sara A. Dyer and Elaine K. Green. The Protein Truncation Test Carol A. Hardy Mutation Detection in Factor VIII cDNA from Lymphocytes of Haemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch Naushin H. Waseem, Richard Bagnall, Peter M. Green, and Francesco Giannelli Denaturing Gradient Gel Electrophoresis Yvonne Wallis Conformation-Sensitive Gel Electrophoresis Ian J. Williams and Anne C. Goodeve SSCP/Heteroduplex Analysis Andrew J. Wallace Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection Laura Heisler and Chao-Hung Lee Automated Genotyping Using the DNA MassArray™ Technology Christian Jurinke, Dirk van den Boom, Charles R. Cantor, and Hubert Köster An Introduction to Bioinformatics Henry Brzeski

Su questa edizione

• Editore: Humana Pr Inc
• Data di pubblicazione: 2002
• ISBN 10: 0896036170
• ISBN 13: 9780896036178
• Rilegatura: Copertina flessibile
• Numero edizione: 1
• Numero di pagine: 224
• Redattore: Theophilus Bimal D. M., Rapley Ralph