SV40 Protocols: 165 - Rilegato

Rilegato

ISBN 10: 0896036537 ISBN 13: 9780896036536

Casa editrice: Springer-Verlag GmbH, 2000

Riassunto

The initial incentive for in-depth study of Simian Virus 40 biology originated from the revelation of public health implications its use had in the 1960s. SV40 gained notoriety at this time when it was found to be a contaminant of polio and adenovirus vaccines that had been distributed to millions of healthy individuals worldwide. SV40 Protocols presents a consolidation of numerous well-tested step-by-step techniques from experts with hands-on experience. The book is organized in two sections. The first relates to assays of the lytic cycle of the virus, starting with a valuable introductory chapter on basic techniques for growth of SV40 and its mutants in tissue culture. Techniques for the growth and detection of two SV40-related human viruses, BKV and JCV, are also presented for those involved in clinical research. The second section begins with detailed biological assays of neoplastic transformation that are applicable to any system. All of the detailed and thorough chapters include discussions of problems and pitfalls that may be encountered, as well as troubleshooting advice. This comprehensive volume of optimzied experimental protocols will assist the beginner, as well as more experience researchers, to over come problems of ambiguity often present in SV40 literature.

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Dalla quarta di copertina

Extensive research has shown that Simian Virus 40, a contaminant of polio and adenovirus vaccines that may be implicated in human cancers, can also serve as a powerful probe for examining many fundamental questions in molecular biology. In SV40 Protocols, Leda Raptis and a panel of highly experienced investigators describe in step-by-step fashion key techniques for experimentally detecting SV40 in human tumors, for exploiting its use in human gene therapy, and for studying its replication and its mechanisms of neoplastic transformation. Included are methods for growing SV40 and its related viruses in tissue culture, for in vivo and in vitro replication and transcription of SV40 DNA, for the use of retroviral vectors to express SV40 tumor antigens in cultured cells, and for transgenic mouse models based on the SV40 large T antigen. All methods have been optimized for experimental success, and the authors provide cogent discussions of the problems and pitfalls that may be encountered, as well as valuable troubleshooting advice. An appendix lists all companies whose products are cited in the text and includes an Internet directory for locating other reagent sources.
Detailed and highly practical, SV40 Protocols offers both clinical and basic researchers powerful, well-tested tools for research on SV40 replication and neoplastic transformation, as well as techniques for its detection in human tumors and for creating and using powerful new gene therapy vectors.

Dettagli dell'edizione

• Editore: Springer-Verlag GmbH
• Data di pubblicazione: 2000
• Lingua: Inglese
• ISBN 10: 0896036537
• ISBN 13: 9780896036536
• Rilegatura: Copertina rigida
• Numero di pagine: 328
• Redattore: Raptis Leda
• Contatto del produttore: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Germania