Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques: 173 - Rilegato

Rilegato

ISBN 10: 0896036898 ISBN 13: 9780896036895

Casa editrice: Springer-Verlag GmbH, 2002

Riassunto

Calcium plays an important role in a wide variety of biological processes and is uniquely suited to act as a promoter or preventer of activities in biological systems. These two volumes provide the modern experimental techniques for studying calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods, and many of the methods described can also be used in the study of proteins that do not bind calcium. Volume II of this two volume set provides a vast array of modern experimental techniques that can be used to study the solution structure, stability, dynamics, calcium-binding properties and biological activity of calcium-binding proteins in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, particular attention is paid to modern NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, as well as fluorescence methods to study the distribution of calcium and calcium binding proteins in cells. In addition, studies of their ligand binding properties and their distribution in cells are included. Volume I of this two-volume set provides information on the role of calcium in intracellular secondary messenger activation mechanisms. It also provides a wealth of information about protein purification strategies, X-ray crystallography and other studies focused on specific calcium-binding proteins. By introducing the various classes of intra- and extra-cellular calcium-binding proteins and their mode of action, this first volume sets the stage and provides the necessary background for Volume II. Together, these two volumes provide all that a researcher needs to conduct research on calcium-binding proteins.

Su questo libro

Dalla quarta di copertina

Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols- Volume 1: Reviews and Case Histories and Volume 2: Methods and Techniques-Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant class proteins, and provide a comprehensive collection of proven experimental techniques for studying it both in vitro and in vivo. This second volume focuses on cutting-edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells. The first companion volume, Reviews and Case Histories sets the stage for this volume by introducing the various classes of intra- and extracellular calcium-binding proteins and their mode of action.
Comprehensive and highly practical, the two volumes of Calcium-Binding Protocols provide experimental and clinical biologists with a host of advanced experimental methods that can be applied successfully to the study of both existing and newly discovered members of this critically important class of proteins.

Dettagli dell'edizione

• Editore: Springer-Verlag GmbH
• Data di pubblicazione: 2002
• Lingua: Inglese
• ISBN 10: 0896036898
• ISBN 13: 9780896036895
• Rilegatura: Copertina rigida
• Numero di pagine: 436
• Redattore: Vogel Hans J.
• Contatto del produttore: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Germania