High Throughput Screening: Methods and Protocols: v. 190 - Rilegato

Rilegato

ISBN 10: 0896038890 ISBN 13: 9780896038899

Casa editrice: Humana Pr Inc, 2002

Riassunto

In High Throughput Screening, leading scientists and researchers expert in molecular discovery explain the diverse technologies and key techniques used in HTS and demonstrate how they can be applied generically. Writing to create precisely the introductory guidebook they wish had been available when they started in HTS, these expert seasoned authors illuminate the HTS process with richly detailed tutorials on the biological techniques involved, the management of compound libraries, and the automation and engineering approaches needed. Extensive discussions provide readers with all those key elements of pharmacology, molecular biology, enzymology, and biochemistry that will ensure the identification of suitable targets and screens, and detail the technology necessary to mine millions of data points for meaningful knowledge.

Su questo libro

Dalla quarta di copertina

Since its first use in antibiotic research in the 1940s, High Throughput Screening (HTS) has evolved into a major enabling tool and methodology in the discovery of pharmaceutical, chemical, and agricultural compounds. In High Throughput Screening, leading scientists and researchers expert in molecular discovery explain the diverse technologies and key techniques used in HTS and demonstrate how they can be applied generically. Writing to create precisely the introductory guidebook they wished had been available when they started in HTS, these seasoned authors illuminate the HTS process with richly detailed tutorials on the biological techniques involved, the management of compound libraries, and the automation and engineering approaches needed. Extensive discussions provide readers with all those key elements of pharmacology, molecular biology, enzymology, and biochemistry that will ensure the identification of suitable targets and screens, and detail the technology necessary to mine millions of data points for meaningful knowledge.
By presenting accounts not only of the basic principles underlying HTS technology, but also real-use cases, High Throughput Screening constitutes a highly practical introduction to HTS, even as it also provides sufficient detail to work more productively in today's lead generation laboratories.

Contenuti

Design and Implementation of High Throughput Screening Assays Ricardo Macarrón and Robert P. Hertzberg Configuring Radioligand Receptor Binding Assays for HTS Using Scintillation Proximity Assay Technology John W. Carpenter, Carmen Laethem, Frederick R. Hubbard, Thomas K. Eckols, Melvyn Baez, Don McClure, David L. G. Nelson, and Paul A. Johnston Homogeneous Techniques for Monitoring Receptor-Ligand Interactions James S. Marks, Douglas S. Burdette, and David A. Giegel Comparison of SPA, FRET, and FP for Kinase Assays Jinzi J. Wu Electrochemiluminescence: A Technology Evaluation and Assay Reformatting of the Stat6/P578 Protein-Peptide Interaction Robert H. Schweitzer and Laura Abriola Cellular Assays in HTS Patricia Johnston Compound Library Management James A. Chan and Juan A. Hueso-Rodríguez Compound Library Management: An Overview of an Automated System Wilma W. Keighley and Terry P. Wood Natural Products or Not? How to Screen for Natural Products in the Emerging HTS Paradigm Susan P. Manly, Ramesh Padmanabha, and Susan E. Lowe Introduction to Screening Automation Steven Hamilton Unit Automation in High Throughput Screening Karl C. Menke Fully Automated Screening Systems Seth Cohen and Robert F. Trinka Management and Maintenance of High Throughput Screening Systems Barbara Hynd

Dettagli dell?edizione

• Editore: Humana Pr Inc
• Data di pubblicazione: 2002
• ISBN 10: 0896038890
• ISBN 13: 9780896038899
• Rilegatura: Copertina rigida
• Lingua: Inglese
• Numero edizione: 1
• Numero di pagine: 265
• Redattore: Janzen William P.