CRISPR Guide RNA Design: Methods and Protocols: 2162 - Brossura

 
9781071606896: CRISPR Guide RNA Design: Methods and Protocols: 2162
Brossura
ISBN 10: 1071606891 ISBN 13: 9781071606896
Casa editrice: Humana, 2021

Sinossi

This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.

Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.

Dalla quarta di copertina

This detailed volume focuses on the CRISPR-associated guide RNA and how it can be designed, modified, and validated for a broad repertoire of purposes. Beginning with a section on computational design of target-specific guide RNAs, the book continues by covering chemical modifications to alter guide RNA stability, specificity, and efficiency, as well as to create inducible guide RNAs, append additional functional domains, and express guide RNAs in a conditional manner. It concludes with methods for measuring off-target guide RNA activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and essential, CRISPR Guide RNA Design: Methods and Protocols provides a comprehensive pipeline for guide RNA design and aims to be an invaluable resource in applying this powerful technology to basic research and therapeutic applications.

Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.

Editore
Humana
Data di pubblicazione
2021
Lingua
Inglese
ISBN 10 
1071606891
ISBN 13 
9781071606896
Rilegatura
Copertina flessibile
Numero edizione
1
Numero di pagine
296
Redattore
Fulga Tudor A., Knapp David J. H. F., Ferry Quentin R. V.
Contatto del produttore
Springer Nature Customer Service Center GmbH
ProductSafety@springernature.com

Europaplatz 3,69115 Heidelberg, Germany
Heidelberg
69115
Germania

Altre edizioni note dello stesso titolo

9781071606865: Crispr Guide Rna Design: Methods and Protocols: 2162

Edizione in evidenza

ISBN 10:  1071606867 ISBN 13:  9781071606865
Casa editrice: Humana Pr Inc, 2020
Rilegato