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Essential Genes and Genomes: Methods and Protocols: 2377 - Rilegato

 
9781071617199: Essential Genes and Genomes: Methods and Protocols: 2377

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This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that have revolutionized the field, the collection begins with chapters employing CRISPR/Cas9-based approaches followed by Tn-seq-based approaches, but later chapters also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for the highly successful <i>Methods in Molecular Biology</i> series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.&nbsp;<div><br></div><div>Comprehensive and authoritative, <i>Essential Genes and Genomes: Methods and Protocols</i> is an ideal guide for researchers attempting to strip genetics down to its fundamentals.</div>

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This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that have revolutionized the field, the collection begins with chapters employing CRISPR/Cas9-based approaches followed by Tn-seq-based approaches, but later chapters also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for the highly successful <i>Methods in Molecular Biology</i> series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.&nbsp;<div><br></div><div>Comprehensive and authoritative, <i>Essential Genes and Genomes: Methods and Protocols</i> is an ideal guide for researchers attempting to strip genetics down to its fundamentals. </div>

Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.