Craniofacial Development: Methods and Protocols: 2403 - Rilegato

Rilegato

ISBN 10: 1071618466 ISBN 13: 9781071618462

Casa editrice: Humana Pr Inc, 2021

Riassunto

<p>This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (<i>c. elegans</i>, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and&nbsp;<i>in vivo</i>&nbsp;cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using&nbsp;<i>in-situ&nbsp;</i>hybridisation and&nbsp;single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as&nbsp;CRISPR/Cas9-mediated deletion.&nbsp;Written in the format of the highly successful&nbsp;<i>Methods in Molecular Biology&nbsp;</i>series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.&nbsp;</p>Authoritative and cutting-edge,&nbsp;<i>Craniofacial Development: Methods and Protocols</i>&nbsp;aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques.&nbsp;<p></p><div><br></div>

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Dalla quarta di copertina

This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (<i>c. elegans</i>, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and&nbsp;<i>in vivo</i>&nbsp;cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using&nbsp;<i>in-situ&nbsp;</i>hybridisation and&nbsp;single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as&nbsp;CRISPR/Cas9-mediated deletion.&nbsp;Written in the format of the highly successful&nbsp;<i>Methods in Molecular Biology&nbsp;</i>series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.&nbsp;<div><br>Authoritative and cutting-edge,&nbsp;<i>Craniofacial Development: Methods and Protocols</i>&nbsp;aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques.&nbsp;<p></p><div><br></div></div>

Dettagli dell'edizione

• Editore: Humana Pr Inc
• Data di pubblicazione: 2021
• Lingua: Inglese
• ISBN 10: 1071618466
• ISBN 13: 9781071618462
• Rilegatura: Copertina rigida
• Numero edizione: 1
• Numero di pagine: 328
• Redattore: Dworkin Sebastian
• Contatto del produttore: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3,69115 Heidelberg, Germany; Heidelberg; 69115; Germania