Cell-Free Gene Expression: Methods and Protocols: 2433 - Brossura

 
9781071620007: Cell-Free Gene Expression: Methods and Protocols: 2433

Sinossi

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications.

Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.

Dalla quarta di copertina

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications.

Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.

Altre edizioni note dello stesso titolo

9781071619971: Cell-free Gene Expression: Methods and Protocols: 2433

Edizione in evidenza

ISBN 10:  1071619977 ISBN 13:  9781071619971
Casa editrice: Humana Pr Inc, 2022
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