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This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, mRNA Decay: Methods and Protocols provides both new and experienced RNA researchers with an inspiring collection of protocols to prompt further investigation of these vital degradation pathways.
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mRNA Decay
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Springer Verlag Gmbh Nov 2025, 2025
ISBN 10: 1071641786
ISBN 13: 9781071641781
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mRNA Decay | Methods and Protocols
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Taschenbuch. Condizione: Neu. mRNA Decay | Methods and Protocols | Maxime Wery | Taschenbuch | Methods in Molecular Biology | xiv | Englisch | 2025 | Springer | EAN 9781071641781 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu. Codice articolo 136124893
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Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, mRNA Decay: Methods and Protocols provides both new and experienced RNA researchers with an inspiring collection of protocols to prompt further investigation of these vital degradation pathways.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 440 pp. Englisch. Codice articolo 9781071641781
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mRNA Decay : Methods and Protocols
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Taschenbuch. Condizione: Neu. Druck auf Anfrage Neuware - Printed after ordering - This detailed volume explores techniques for researching the diverse and specialized mechanisms for mRNA degradation, both in the cytoplasm and the nucleus. From classical methods for studying RNA degradation at the single RNA level to the latest transcriptome-wide approaches involving long-read sequencing and metabolic labeling, this book focuses on methods for eukaryotic models, such as procedures for studying deadenylation, decapping and exoribonuclease activity, assessing RNA decay rate, characterizing RNA degradation intermediates, RNA-proteins interactions, and more. Written for the highly successful Methods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, mRNA Decay: Methods and Protocolsprovides both new and experienced RNA researchers with an inspiring collection of protocols to prompt further investigation of these vital degradation pathways. Codice articolo 9781071641781
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