Proteomics of Biological Systems: Protein Phosphorylation Using Mass Spectrometry Techniques - Rilegato

Ham, Bryan M.

Rilegato

ISBN 10: 1118028961 ISBN 13: 9781118028964

Casa editrice: John Wiley & Sons Inc, 2011

Riassunto

Phosphorylation is the addition of a phosphate (PO₄) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies.

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Informazioni sull?autore

BRYAN M. HAM, PhD, is a member of the American Society for Mass Spectrometry and the American Chemical Society. He has conducted proteomics and lipidomics research at The Ohio State University and Pacific Northwest National Laboratory in Richland, Washington. He is currently working for the Department of Homeland Security at the U.S. Customs and Border Protection New York Laboratory. He has published numerous research papers in peer-reviewed journals, and is the author of Even Electron Mass Spectrometry with Biomolecule Applications (Wiley).

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Obtain protein phosphorylation knowledge using mass spectrometry techniques

A practical introduction to mass spectrometry?based phosphoproteomic studies, this book addresses various concepts including the protocols necessary for sample preparation through mass spectral interpretation of protein phosphorylation events. It delves into the determining factors for whether or not a protein has been correctly modified by the addition of a phosphate group. Highlighting both basic and significant principles, as well as specific applications using examples, Proteomics of Biological Systems covers:

• Key fundamentals of mass spectrometry including mass analyzers, ionization, and nano-electrospray/nano-flow liquid chromatography

• Optimized methodologies for performing post translational modification (PTM) of protein studies using tried and tested sample preparation and analysis methods and approaches

• Specific examples of eukaryote and prokaryote normal state and perturbed state phosphoproteomic studies

• Use of the most recent systems biology bioinformatic Internet tools for interpreting complex data such as the Blast2GO gene ontology tool

• Step-by-step methodology for phosphoproteomic studies of both eukaryote and prokaryote systems

This unique resource contains specific chapters devoted to normal state HeLa cell phosphoproteomes, DNA damage response state HeLa cell phosphoproteomes, Caulobacter crescentus normal state phosphoproteomes, and more. A core guide for scientists and researchers in academic laboratories and pharmaceutical, chemical, and biotech companies, Proteomics of Biological Systems is a useful text for expanding comprehension of mass spectrometry?based phosphoproteomic study.

Dettagli dell'edizione

• Editore: John Wiley & Sons Inc
• Data di pubblicazione: 2011
• Lingua: Inglese
• ISBN 10: 1118028961
• ISBN 13: 9781118028964
• Rilegatura: Copertina rigida
• Numero di pagine: 350
• Contatto del produttore: non disponibile
• Persona responsabile: non disponibile