Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function. Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come.
Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.
1. Evolving Mechanistic Insights into Galectin Functions
Connie M. Arthur, Marcelo Dias-Baruffi, Richard D. Cummings, and Sean R. Stowell
2. Cloning, Expression and Purification of Galectins for In Vitro Studies
Paul A. Poland, Carol L Kinlough, and Rebecca P. Hughey
3. Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation
Sean R. Stowell, Connie M. Arthur, Richard D. Cummings, and Christa L. Feasley
4. Evaluation of Galectin Binding by Frontal Affinity Chromatography (FAC)
Jun Iwaki and Jun Hirabayashi
5. Probing Lectin-Mucin Interactions by Isothermal Titration Microcalorimetry
Tarun K. Dam and C. Fred Brewer
6. Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis
Anne Leppänen, Connie M. Arthur, Sean R. Stowell, Richard D. Cummings
7. Evaluation of Galectin Binding by Surface Plasmon Resonance
Padmaja Mehta-D’souza
8. Examining Galectin Binding Specificity Using Glycan Microarrays
Connie M. Arthur, Lilian C Rodrigues, Marcelo Dias-Baruffi, Harold C. Sullivan, Jamie Heimburg-Molinaro, Dave F. Smith, Richard D. Cummings, and Sean R. Stowell
9. Methods for Assessing the Effects of Galectins on Leukocyte Trafficking Beatrice R. Gittens, Rachael D. Wright, and Dianne Cooper
10. Examination of the Role of Galectins in Plasma Cell Differentiation
Chih-Ming Tsai and Kuo-I Lin
11. Examination of Galectin-Induced Lattice Formation on Early B-Cell Development
Stéphane J.C. Mancini, Latifa Elantak, Annie Boned, Marion Espéli, Françoise Guerlesquin, and Claudine Schiff
12. Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins
Connie M. Arthur, Lilian C Rodrigues, Marcelo Dias-Baruffi, Harold C. Sullivan Richard D. Cummings, and Sean R. Stowell
13. Examination of Galectins in Phagocytosis
Huan-Yuan Chen, I-Chun Weng, Chi-Shan Li, Lei Wan, and Fu-Tong Liu
14. Assessing the Roles of Galectins in Regulating Dendritic Cell Migration Through Extracellular Matrix and Across Lymphatic Endothelial Cells
Sandra Thiemann, Jeanette H. Man, and Linda G. Baum
15. Examination of the Role of Galectins in Intestinal Inflammation
Atsushi Nishida, Cindy W. Lau, and Atsushi Mizoguchi
16. Study of Galectins in Tumor Immunity: Strategies and Methods
Juan P. Cerliani, Tomas Dalotto-Moreno, Daniel Compagno, L. Sebastián Dergan-Dylon, Diego J. Laderach, Lucas Gentilini, Diego O. Croci, Santiago P. Méndez-Huergo, Marta A. Toscano, Mariana Salatino, and Gabriel A. Rabinovich
17. Galectins in the Regulation of Platelet Biology
Albertina Romaniuk, Gabriel A Rabinovich, and Mirta Schattner
18. Examination of the Role of Galectins and Galectin Inhibitors in Endothelial Cell Biology
Iris A.E. Schulkens, Esther A. Kleibeuker, Kitty C.M. Castricum, Arjan W. Griffioen, and Victor L.J.L. Thijssen
19. Regulation of Galectins by Hypoxia and Their Relevance in Angiogenesis: Strategies and Methods
Mariana Salatino, Diego O. Croci, Diego J. Laderach, Daniel Compagno, Lucas Gentilini, Tomas Dalotto-Moreno, L. Sebastián Dergan-Dylon, Santiago P. Méndez-Huergo, Marta A. Toscano, Juan P. Cerliani and Gabriel A. Rabinovich
20. Examination of the Role of Galectins During In vivo Angiogenesis Using the Chick Chorioallantoic Membrane Assay
Esther A. Kleibeuker, Iris A. Schulkens, Kitty C.M. Castricum, Arjan W. Griffioen, Victor L.J.L. Thijssen
21. Examination of the Role of Galectins in Cell Migration and Re-Epithelialization of Wounds
Zhiyi Cao, Chandrassegar Saravanan, Wei-Sheng Chen, and Noorjahan Panjwani
22. Manipulating Galectin Expression in Zebrafish (Danio rerio)
Chiguang Feng, Mihai Nita-Lazar, Nuria González-Montalbán, Jingyu Wang, Justin Mancini, Chinnarajan Ravindran, Hafiz Ahmed, and Gerardo R. Vasta
23. Examination of Galectin Localization Using Confocal Microscopy
Daniel Giuliano Cerri, Connie M. Arthur, Lílian Cataldi Rodrigues, Marise Lopes Fermino, Lenaldo Branco Rocha, Sean R. Stowell, and Marcelo Dias-Baruffi
24. Examination of the Regulation of Galectin-3 Expression in Cancer
Hafiz Ahmed and Gargi Bandyopadhyaya
25. Evaluation of the Role of Galectins in Parasite Immunity
Sarah Preston, Jillian Dunphy, Travis Beddoe, Els Meeusen, and Anna Young
26. Effect of Galectins on Viral Transmission
Michel Ouellet, Christian St-Pierre, Michel J. Tremblay, and Sachiko Sato
27. Evaluation of the Bactericidal Activity of Galectins
Connie M. Arthur, Richard D. Cummings, and Sean R. Stowell
28. Examination of the Role of Galectins in Pre-mRNA Splicing
Ronald J. Patterson, Kevin C. Haudek, Patricia G. Voss, and John L. Wang
29. Analysis of the Intracellular Role of Galectins in Cell Growth and Apoptosis
Daniel K. Jsu, Ri-Yao Yang, Jun Saegusa, and Fu-Tong Liu
30. Nucleocytoplasmic Shuttling of Galectin-3
Eric J. Arnoys, Cheri M. Ackerman, and John L. Wang
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Descrizione libro Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the expertsIncludes cutting-edge methods and protocolsProvides s. Codice articolo 4214113
Descrizione libro Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function. Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come. 504 pp. Englisch. Codice articolo 9781493913954
Descrizione libro Buch. Condizione: Neu. Druck auf Anfrage Neuware - Printed after ordering - Galectins: Methods and Protocols is the first book solely dedicated to methodological approaches designed to study galectin function. The galectin family represents one of the most pleiotropic families, with individual members having been implicated in various aspects of nearly every biological process described, from RNA splicing to complex regulatory circuits that orchestrate adaptive immunity. Given the diverse roles of galectins in a variety of biological systems, studying these glycan binding proteins often requires the assimilation of diverse technical skills to fully appreciate their biological function. Their nearly ubiquitous expression and ability to bind highly modifiable carbohydrate ligands, in addition to a variety of other regulatory proteins, allows these glycan binding proteins (GBPs) to possess the capacity to regulate a wide variety of biological processes. Individual chapters are dedicated to examining salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Galectins: Methods and Protocols seeks to serve both professionals and novices with a useful framework when examining galectin function for many years to come. Codice articolo 9781493913954
Descrizione libro Condizione: New. 2014. Hardcover. . . . . . Codice articolo V9781493913954
Descrizione libro Hardcover. Condizione: Brand New. 488 pages. 10.50x7.25x1.50 inches. In Stock. Codice articolo x-1493913956