In Vitro Mutagenesis: Methods and Protocols: 1498 - Brossura

Brossura

ISBN 10: 1493982117 ISBN 13: 9781493982110

Casa editrice: Humana Pr Inc, 2018

Riassunto

In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.

Dettagli dell'edizione

• Editore: Humana Pr Inc
• Data di pubblicazione: 2018
• Lingua: Inglese
• ISBN 10: 1493982117
• ISBN 13: 9781493982110
• Rilegatura: Copertina flessibile
• Numero di pagine: 528
• Redattore: Reeves Andrew
• Contatto del produttore: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Germania