Progenitor Cells: Methods and Protocols: 2029 - Brossura

Brossura

ISBN 10: 1493996339 ISBN 13: 9781493996339

Casa editrice: Humana, 2020

Riassunto

This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton’s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Progenitor Cells: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field. 

Su questo libro

Dalla quarta di copertina

This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Progenitor Cells: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field. 

Dettagli dell'edizione

• Editore: Humana
• Data di pubblicazione: 2020
• Lingua: Inglese
• ISBN 10: 1493996339
• ISBN 13: 9781493996339
• Rilegatura: Copertina flessibile
• Numero edizione: 1
• Numero di pagine: 304
• Redattore: Joglekar Mugdha V., Hardikar Anandwardhan A.
• Contatto del produttore: non disponibile
• Persona responsabile: ProductSafety@springernature.com; ProductSafety@springernature.com; ; Poststr. 9; Darmstadt; 64293; Germania