Articoli correlati a Handbook of ELISPOT: Methods and Protocols: v. 302

Handbook of ELISPOT: Methods and Protocols: v. 302 - Rilegato

 
9781588294692: Handbook of ELISPOT: Methods and Protocols: v. 302
Rilegato
ISBN 10: 1588294692 ISBN 13: 9781588294692
Casa editrice: Humana Pr Inc, 2005

Sinossi

In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.

Dalla quarta di copertina

Although the ELISPOT (enzyme-linked immunospot) assay is widely used in many fields of biomedical research, producing recognizable and quantifiable spots may be very difficult not only for beginners, but also for experienced researchers. In Handbook of ELISPOT: Methods and Protocols, the first book dedicated entirely to the ELISPOT, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Handbook of ELISPOT: Methods and Protocols offers both novice and experienced researchers a wealth of easy-to-understand information that will clarify the technically challenging aspects of ELISPOT and open the door to its wider successful use.

Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.

Editore
Humana Pr Inc
Data di pubblicazione
2005
Lingua
Inglese
ISBN 10 
1588294692
ISBN 13 
9781588294692
Rilegatura
Copertina rigida
Numero di pagine
321
Redattore
Kalyuzhny Alexander E.
Contatto del produttore
non disponibile
Persona responsabile
non disponibile