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The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production.
The chapters are written by world-renowned experts and the volume’s five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.
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Contenuti
Animal Cell Biotechnology - Methods and Protocols Editor: Ralf Pörtner Contents Preface Contributors Part I. Basic techniques for establishing production cell lines 1 Transduction Technologies David A. Fluri and Martin Fussenegger 2 Cells for gene therapy and vector production C. Delenda, M. Chillon, A.-M. Douar and Otto-Wilhelm Merten 3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments Alexandra Dorn-Beineke, Stefanie Nittka and Michael Neumaier 4 DNA Fingerprinting and Characterisation of Animal Cell Lines Glyn N. Stacey, Ed Byrne and J. Ross Hawkins Part II. Basic cultivation techniques 5 Microcarrier Cell culture Technology Gerald Blüml 6 Cell Encapsulation Holger Hübner 7 Tools for High-Throughput Medium and Process Optimisation Martin Jordan and Nigel Jenkins Part III. Cell characterisation and analysis 8 Cell counting and viability measurements Michael Butler and Maureen Spearman 9 Monitoring of Growth, Physiology and Productivity of animal cells by Flow Cytometry Silvia Carroll, Mariam Nasiri and Mohamed Al-Rubeai 10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors André A. Neves and Kevin M. Brindle 11 Methods for off-line analysis of nutrients and products in mammalian cell culture Heino Büntemeyer 12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation Derek Adams, Rashmi Korke, and Wei-Shou Hu 13 Measurement of apoptosis in cell culture Adiba Ishaque1 and Mohamed Al-Rubeai 14 Metabolic Flux Estimation in Mammalian Cell Cultures Chetan T. Goudar, Richard Biener, JamesM. Piret and Konstantin B. Konstantinov Part IV. Special cultivation techniques 15 Disposable bioreactors for inoculum production and protein expression Regine Eibl and Dieter Eibl 16 Hollow Fibre Cell Culture John M. Davis 17 Cultivation of mammalian cells in fixed bed reactors Ralf Pörtner and Oscar B.J. Platas Barradas 18 Configuration of Bioreactors Dirk E. Martens and Evert Jan van den End Part V. Downstream techniques 19 Membrane filtration in animal cell culture Peter Czermak, Dirk Nehring and Ranil Wickramasinghe 20 Chromatographic techniques in the downstream processing of (recombinant) proteins Ruth Freitag Part VI. Special applications 21 Vaccine production - state of the art and future needs in upstream processing Yvonne Genzel and Udo Reichl 22 Retrovirus production and characterization Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues, Paula Alves 23 Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production Sabine Geisse Index
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Animal Cell Biotechnology: Methods and Protocols (Methods in Biotechnology) (Volume 24) (Second Edition)
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Condizione: Good. Volume 24. This is an ex-library book and may have the usual library/used-book markings inside.This book has hardback covers. Clean from markings In good all round condition. No dust jacket. Please note the Image in this listing is a stock photo and may not match the covers of the actual item,1150grams, ISBN:9781588296603. Codice articolo 9439868
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Hardcover. Condizione: new. Hardcover. Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes. Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. Shipping may be from multiple locations in the US or from the UK, depending on stock availability. Codice articolo 9781588296603
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Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The chapters are written by world-renowned experts and the volume's five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes. 532 pp. Englisch. Codice articolo 9781588296603
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Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Chapters written by world-renowned expertsDetails novel techniques for basic cultivation and complex techniques such as disposable bioreactors, hollow fiber cell culture and fixed bed reactorsProvides a state-of-the-art manual for setting u. Codice articolo 458519528
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Buch. Condizione: Neu. Neuware -Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation.Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors.Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 532 pp. Englisch. Codice articolo 9781588296603
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Animal Cell Biotechnology: Methods and Protocols
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Condizione: New. This book is a compendium of novel techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes. The volume's five parts reflect the processes required for different stages of production. Editor(s): Portner, Ralf. Series: Methods in Biotechnology. Num Pages: 512 pages, 31 black & white tables, biography. BIC Classification: TCB. Category: (P) Professional & Vocational. Dimension: 235 x 155 x 28. Weight in Grams: 1018. . 2007. 2nd ed. 2007. hardcover. . . . . Codice articolo V9781588296603
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