Arthritis Research: Volume 1: Methods and Protocols: 135 - Brossura

Brossura

ISBN 10: 1617375071 ISBN 13: 9781617375071

Casa editrice: Humana, 2010

Riassunto

Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover such exciting and cutting edge topics as imaging and immunohistochemistry, analysis of cartilage and bone catabolism, immunobiology, and cell trafficking.

In Volume 1, authors discuss synovial joint morphology, histopathology, and immunohistochemistry, cartilage matrix and bone biology, and cell trafficking, migration and invasion. Volume 2 is broken up into sections that cover immunobiology and T cells, animal models of arthritis, and applications of new technologies, such as Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR), to define novel therapeutic targets. Both volumes combine to produce a concise set of protocols condensing decades of experience and expertise.

Arthritis Research: Methods and Protocols will be a valuable tool for basic research investigators, clinician scientists, pathologists, immunologists, and biochemists looking to stay current in their fields.

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Recensione

From the reviews:

"...offers a comprehensive reference for the analysis of tissues relevant to arthritis research in brief chapters that emphasize how various analyses are performed.  What separates this series from other references in this area is its ability to be brief but thorough in the discussion of technically challenging methods." -Doody's Book Review, Weighted Numerical Score:95 - 4 Stars

"This multiauthored ... compendium of arthritis research methods and protocols provides pertinent practical information for the bench researcher as well as for the interested clinician. ... It is a worthwhile reference to have in the research laboratory ... . I enthusiastically recommend ... and believe this publication would have broad appeal to the rheumatologic community and to those in related specialties. ... I congratulate the authors on developing an excellent compendium of arthritis research ... ." (Robert J. Quinet, JAMA, April, 2008)

"Arthritis Research: Methods and Protocols forms part of a book series on Methods in Molecular Medicine by Humana Press. ... Overall the books are extremely useful as both a practical guide and a reference text and are easy to understand. We would recommend the books to anyone with an interest in rheumatology research – basic scientists, clinicians and students ... ." (Leonie Taams and Hayley Evans, Immunology News, August, 2008)

Contenuti

Arthritis Research: Methods and Protocols, Volume I Andrew P. Cope, PhD I. Synovial Joint Morphology, Histopathology, and Immunohistochemistry 1. Imaging Inflamed Synovial Joints Ai Lyn Tan, Helen I. Keen, Paul Emery, and Dennis McGonagle 2. Arthroscopy as a Research Tool: A Review Richard J. Reece 3. Immunohitochemistry of the Inflamed Synovium Martina Gogarty and Oliver FitzGerald 4. In Situ Hybridisation of Synovial Tissue Stefan Kuchen, Christian A. See,ayer, Michel Neidhart, Renate E. Gay, and Steffen Gay 5. Subtractive Hybridization Jörg H. W. Distler, Oliver Distler, Michel Neidhart, and Steffen Gay 6. Laser Capture as a Tool for Analysis of Gene Expression in Inflamed Synovium Ulf Müller-Ladner, Martin Judex, Elena Neumann and Steffen Gay 7. Preparation of Mononuclear Cells from Synovial Tissue Jonathan T. Beech and Fionula M. Brennan 8. Quantitative Image Analysis of Synovial Tissue Pascal O. van der Hall, Maarten C. Kraan and Paul Peter Tak II. Cartilage Matrix and Bone Biology 9. Cartilage Histomorphometry Ernst B. Hunziker 10. Image Analysis of Aggrecan Degradation in Articular Cartilage with Formalin-Fixed Samples Barbara Osborn, Yun Bai, Anna H.K. Plaas, and John D. Sandy 11. In Situ Detection of Cell Death in Articular Cartilage Samantha N. Redman, Ilyas M. Khan, Simon R. Tew and Charles W. Archer 12. Measurement of Glycosaminoglycan Release from Cartilage Explants John S. Mort and Peter J. Roughley 13. Assessment of Collagenase Activity in Cartilage Tim E.Cawston and Tanya G. Morgan 14. Assessment of Gelatinase Expression and Activity in Articular Cartilage Rosalind M. Hembry, Susan J. Atkinson and Gillian Murphy 15. Analysis of MT1-MMP Activity Richard D. Evans and YoshifumiItoh 16. Analysis of TIMP Expression and Activity Linda Troeberg and Hideaki Nagase 17. Bone Histomorphology in Arthritis Models Georg Schett and Birgit Tuerk 18. Generation of Osteoclasts in vitro and Assay of Osteoclast Activity Naoyuki Takahashi, Nobuyuki Udagawa, Yasuhiro Kobayashi and Tatsuo Suda III. Cell Trafficking, Migration and Invasion 19. Isolation and Analysis of Large and Small Vessel Endothelial Cells Justin C. Mason, Elaine A Lidington and Helen Yarwood 20. Analysis of Flow Based Adhesion in vitro Oliver Florey and Dorian O. Haskard 21. Analysis of Leukocyte Recruitment in Synovial Microcirculation by Intravital Microscopy Gabriela Constantin 22. Angiogenesis in Arthritis - Methodological and Analytical Details Ursula Fearon and Douglas J. Veale 23. Analysis of Inflammatory Leukocyte and Endothelial Chemotactic Activity Zoltan Szekanecz and Alisa Koch 24. Acquisition, Culture and Phenotyping of Synovial Fibroblasts Sanna Rosengren, David L. Boyle and Gary S. Firestein 25. Genotyping of Synovial Fibroblasts: cDNA Array in Combination with RAP-PCR in Arthritis Elena Neumann, Martin Judex, Steffen Gay and Ulf Müller-Ladner 26. Gene Transfer to Synovial Fibroblast - Methods and Evaluation in the SCID Mouse Model Ingmar Meinecke, Edita Rutkauskaite, Antje Cinski, Ulf Müller-Ladner, Steffen Gay and Thomas Pap 27. In vitro Matrigel Fibroblast Invasion Assay Tanja C.A. Tolboom and Tom W.J. Huizinga 28. Culture and Analysis of Circulating Fibrocytes Timothy E. Quan and Richard Bucala

Dettagli dell'edizione

• Editore: Humana
• Data di pubblicazione: 2010
• Lingua: Inglese
• ISBN 10: 1617375071
• ISBN 13: 9781617375071
• Rilegatura: Copertina flessibile
• Numero di pagine: 468
• Redattore: Cope Andrew P.
• Contatto del produttore: Springer Nature Customer Service Center GmbH; ProductSafety@springernature.com; ; Europaplatz 3; Heidelberg; 69115; Germania