Single Cell Diagnostics: Methods and Protocols: 132 - Brossura

Brossura

ISBN 10: 161737654X ISBN 13: 9781617376542

Casa editrice: Humana, 2010

Riassunto

The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.

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Recensione

From the reviews:

"Single cell diagnostics has become an increasingly important field for both clinicians and researchers who are involved in infertility treatment. ... For researchers this book provides not only detailed state of the art protocols for single cell diagnostics, but also valuable notes on troubleshooting and pitfalls. For clinicians in the field of infertility this nice overview gives an insight into single cell diagnostics and its scientific background. ... This book can be warmly recommended to clinicians and researchers caring for patients with fertility problems." (G. Haidl and J. P. Allam, Andrologia, Vol. 40 (1), 2008)

"This book details innovative methods for analyzing gene expression, chromosome organization, and proteins in single cells and cell extracts. ... The book is written for basic science and clinical researchers, particularly those providing care to couples seeking advice regarding infertility and inherited genetic disorders. ... The chapters are written by experts and the book is carefully edited. ... The laboratory protocols and tips will be valued by laboratory scientists interested in forensic medicine, reproductive medicine, and developmental genetics." (Bruce A. Fenderson, Doody’s Review Service, October, 2007)

Contenuti

Table of Contents 1. Non-invasive metabolic assessment of single cells David K. Gardner 2. Laser Capture Microdissection for Analysis of Single Cells Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, and Ricardo V. Lloyd 3. Fluorescence in situ hybridization (FISH) on Single Cells (Sex Determination and Chromosome Rearrangements) Paul N. Scriven and Caroline Mackie Ogilvie 4. Single cell PCR for PGD: methods, strategies and limitations Karen Sermon and Martine De Rycke 5. Real-Time Quantitative PCR Measurement of Male Fetal DNA in maternal plasma Bernhard Zimmermann, Ahmad El-Sheikhah, Kypros Nicolaides, Wolfgang Holzgreve, Sinuhe Hahn 6. Cell-Free Fetal DNA Plasma Extraction and Real Time-PCR Quantification Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchi 7. LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cells Kenneth E. Pierce and Lawrence J. Wangh 8. Efficient isothermal amplification of the entire genome from single cells Karen V. Schowalter, Jolene R. Fredrickson and Alan R Thornhill 9. Comparative Genomic Hybridization on single cells Lucille Voullaire and Leeanda Wilton 10. Generation of amplified RNAs and cDNA libraries from single mammalian cells James Adjaye 11. Real-time PCR to measure gene expression in single cells Dagan Wells 12. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridisation (Array CGH) Dong Gui Hui, Xin Yuan Guan, and Nicole Hussey 13. Microarray technology for mutation analysis of low template DNA samples Chelsea Salvado and David Cram

Dettagli dell'edizione

• Editore: Humana
• Data di pubblicazione: 2010
• Lingua: Inglese
• ISBN 10: 161737654X
• ISBN 13: 9781617376542
• Rilegatura: Copertina flessibile
• Numero di pagine: 196
• Redattore: Thornhill Alan R.
• Contatto del produttore: non disponibile
• Persona responsabile: gpsr@libri.de; gpsr@libri.de; ; Friedensallee 273; Hamburg; 22763; Germania