Comparative Transcriptome Analysis using cDNA–AFLP Technique - Brossura

 
9783330001664: Comparative Transcriptome Analysis using cDNA–AFLP Technique
Brossura
ISBN 10: 3330001666 ISBN 13: 9783330001664
Casa editrice: LAP LAMBERT Academic Publishing, 2019

Sinossi

An increased understanding of the molecular mechanisms involved in establishment and development of entomopathogenic fungi can be regarded as an important step towards their effective future insect pest management. To this end, I have successfully applied two different PCR-based approaches – cDNA-AFLPs and Quantitative real-time PCR analysis to identify B. bassiana genes putatively involved in overcoming host insect specific barriers. The cDNA-AFLP display proved to be a very powerful transcriptome-wide gene discovery tool in B. bassiana just as with many other organisms in which this approach was used. Without any previous sequence information, it was possible to gain first insights into the molecular processes endowing metabolic versatility to B. bassiana. However, gene knockout experiments have to be conducted to confirm their putative role in various metabolic pathways. Understanding the interconnection between nitrogen metabolism repression and absence of diverse subtilisin like proteases during growth on insect cuticle is pivotal to understand the metabolic versatility of B. bassiana.

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Editore
LAP LAMBERT Academic Publishing
Data di pubblicazione
2019
Lingua
Inglese
ISBN 10 
3330001666
ISBN 13 
9783330001664
Rilegatura
Copertina flessibile
Numero di pagine
160
Redattore
Pathan Akbar Ali Khan
Contatto del produttore
non disponibile
Persona responsabile
KreGa Onlinehandel GmbH
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info@krega-onlinehandel.de

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