Antibody Engineering - Brossura

Brossura

ISBN 10: 3540413545 ISBN 13: 9783540413547

Casa editrice: Springer Verlag, 2001

Riassunto

Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more. This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered.

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Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more.
This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered. In particular, protocols on the following topics are provided:
Hybridoma immortalisation Generation and screening of antibody gene libraries from human donors, mice and rabbits Antibody selection on immunotubes, cells, tissues; proximity and step-back selections Creation of human monoclonal antibodies to toxic or highly pathogenic agents without immunisation Improvment of antibody binding Antibody humanisation Genetic fusions for the production of multifunctional antibody derivatives Radiolabelled recombinant antibodies Bispecific antibodies Antibody - enzyme fusions Intracellular antibodies Determination of affinity and specificity Computer analysis of antibody sequence and structure Epitope analysis by various phage display systems and peptide spot membranes Eukaryotic (plant, baculovirus, yeast, mammalian cells) and prokaryotic production systems for recombinant antibodies Purification systems Xenograft mice Emerging technologies

Dettagli dell'edizione

• Editore: Springer Verlag
• Data di pubblicazione: 2001
• Lingua: Inglese
• ISBN 10: 3540413545
• ISBN 13: 9783540413547
• Rilegatura: Copertina flessibile
• Numero di pagine: 790
• Redattore: Kontermann Roland, Dubel Stefan
• Contatto del produttore: non disponibile
• Persona responsabile: CMN Printpool Inh. Mathis Neumann; https://www.also.com/ec/cms5/en_6000/6000/index.jsp; ; Friedrich-Karl-Str. 9; Hamburg; Germania