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Simple DNA Extraction Methods from Human Blood: A Comparison of Phenol - Chloroform -Isoamyle, Saturated Sodium Chloride,Guanidine Hydrochloride and ... for DNA Extraction from Human Blood Samples - Brossura
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This study was aimed to compare between four DNA extraction methods, including phenol-chloroform-isoamyle, guanidine hydrochloride, saturated sodium chloride, and chelex-100 using 50 blood samples collected from healthy volunteers. The comparison included DNA concentration, extracted DNA purity, cost of reagents per one sample, and time consuming. After a comprehensive analysis of all factors: salt extraction gave the maximum yield of DNA, it is saver and simpler than the other methods. Moreover, this method is reliable and inexpensive. But its purity is slightly low. Phenol-chloroform-isoamyl extraction method give reliable quantity and purity of the DNA extracted. But it takes a time and labor in obtaining pure extract. Moreover, its associated toxicities warrant a judicious use. Chelex-100 resin extractions gave a DNA quantity as well as phenol-chloroform- isoamyle extraction. It is save, simple, does not require any organic solvent, and takes very short time and a little possibility of cross contamination. But it is expensive. Guanidine hydrochloride extraction is an expensive method taking a long time with a low quantity and quality of the DNA obtained.
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L'autore
Lecturer, Faculty of Medical Laboratory Sciences, Alneelain University, Khartoum, Sudan.M.Sc. in Parasitology and Medical Entomology - Alneelain University. (Jul-2011).Bachelor Degree in Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan (Oct-2004).
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Simple DNA Extraction Methods from Human Blood
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Simple DNA Extraction Methods from Human Blood A Comparison of Phenol Chloroform Isoamyle, Saturated Sodium Chloride,Guanidine Hydrochloride and for DNA Extraction from Human Blood Samples
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Simple DNA Extraction Methods from Human Blood A Comparison of Phenol Chloroform Isoamyle, Saturated Sodium Chloride,Guanidine Hydrochloride and for DNA Extraction from Human Blood Samples
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Simple DNA Extraction Methods from Human Blood
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Simple DNA Extraction Methods from Human Blood
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LAP LAMBERT Academic Publishing Apr 2014, 2014
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Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This study was aimed to compare between four DNA extraction methods, including phenol-chloroform-isoamyle, guanidine hydrochloride, saturated sodium chloride, and chelex-100 using 50 blood samples collected from healthy volunteers. The comparison included DNA concentration, extracted DNA purity, cost of reagents per one sample, and time consuming. After a comprehensive analysis of all factors: salt extraction gave the maximum yield of DNA, it is saver and simpler than the other methods. Moreover, this method is reliable and inexpensive. But its purity is slightly low. Phenol-chloroform-isoamyl extraction method give reliable quantity and purity of the DNA extracted. But it takes a time and labor in obtaining pure extract. Moreover, its associated toxicities warrant a judicious use. Chelex-100 resin extractions gave a DNA quantity as well as phenol-chloroform- isoamyle extraction. It is save, simple, does not require any organic solvent, and takes very short time and a little possibility of cross contamination. But it is expensive. Guanidine hydrochloride extraction is an expensive method taking a long time with a low quantity and quality of the DNA obtained. 56 pp. Englisch. Codice articolo 9783659536342
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Simple DNA Extraction Methods from Human Blood
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Simple DNA Extraction Methods from Human Blood
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Simple DNA Extraction Methods from Human Blood
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VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2014
ISBN 10: 3659536342
ISBN 13: 9783659536342
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Simple DNA Extraction Methods from Human Blood : A Comparison of Phenol - Chloroform -Isoamyle, Saturated Sodium Chloride,Guanidine Hydrochloride and Chelex-100 for DNA Extraction from Human Blood Samples
Editore:
LAP LAMBERT Academic Publishing, 2014
ISBN 10: 3659536342
ISBN 13: 9783659536342
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Taschenbuch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This study was aimed to compare between four DNA extraction methods, including phenol-chloroform-isoamyle, guanidine hydrochloride, saturated sodium chloride, and chelex-100 using 50 blood samples collected from healthy volunteers. The comparison included DNA concentration, extracted DNA purity, cost of reagents per one sample, and time consuming. After a comprehensive analysis of all factors: salt extraction gave the maximum yield of DNA, it is saver and simpler than the other methods. Moreover, this method is reliable and inexpensive. But its purity is slightly low. Phenol-chloroform-isoamyl extraction method give reliable quantity and purity of the DNA extracted. But it takes a time and labor in obtaining pure extract. Moreover, its associated toxicities warrant a judicious use. Chelex-100 resin extractions gave a DNA quantity as well as phenol-chloroform- isoamyle extraction. It is save, simple, does not require any organic solvent, and takes very short time and a little possibility of cross contamination. But it is expensive. Guanidine hydrochloride extraction is an expensive method taking a long time with a low quantity and quality of the DNA obtained. Codice articolo 9783659536342
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Simple DNA Extraction Methods from Human Blood
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ISBN 13: 9783659536342
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Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Ali SamarLecturer, Faculty of Medical Laboratory Sciences, Alneelain University, Khartoum, Sudan.M.Sc. in Parasitology and Medical Entomology - Alneelain University. (Jul-2011).Bachelor Degree in Medical Laboratory Sciences, Universi. Codice articolo 5163156
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