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Sinossi
The metabolism of all living organisms consists of elaborate and complex interactions among numbers of biomolecules, including protein-protein interactions. Biacore is a new device that uses surface plasmon resonance for kinetic analysis of molecular interactions and automates whole analytical processes. The number of users of Biacore is increasing worldwide, but until now nothing had been published in English to explain the principles of this new methodology and the kinds of applications it makes available. This updated and revised version of the Japanese original explains the underlying principles of Biacore, providing concrete examples that utilize the methodology for analysis in areas ranging from basic to applied sciences. With its generous use of illustrations, this book is a valuable source of information for all users of Biacore.
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Contenuti
(1) Significance of the Real-Time Analysis of Biological Interactions.- (2) General Principles: Principles of Surface Plasmon Resonance. Principles of BIACORE.- (3) Basic Procedures: Experimental Procedures. Data Analysis Methods.- (4) Applications: Protein-Protein Interactions. Peptid-Protein Interactions. DNA-Protein Interactions. DNA-DNA Interactions. Sugar-Protein Interactions. Lipid-Protein Interactions. Applications for Purification and Screening. Clinical Applications. Combinations with Other Methods.- (5) The View for the Future.
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Real-Time Analysis of Biomolecular Interactions
Print on DemandDa: moluna, Greven, Germania
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Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. The first book to describe how to use BIACORE, an instrument used by many biochemistsThis book gives various examples for analysis of molecular interactionsUse of many figures makes it easy for readers to understand(1) Significance of the Real-T. Codice articolo 5753290
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Real-Time Analysis of Biomolecular Interactions
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Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Elaborate and complex interactions among the molecules that make up all living or ganisms are the basis by which those organisms carry out their existence. All metabo lism or vital activities of living organisms are attributed to the interactions of numbers of biomolecules, including protein-protein interactions. Cell growth and differentia tion, development and generation or regeneration of tissue, and the occurrence of or protection against various diseases are triggered, conducted, and maintained by the complex sequential interactions among molecules both inside and outside the cells. Each protein has its own function, but it cannot function by itself. For example, enzymes catalyze reactions only through transient interaction with their substrates. In some cases, proteins carry out their functions only after they form a multi subunit com plex. GenerallY, proteins, nucleotides, lipids, sugars, and other small molecules func tion through the interaction of mUltiple molecules either simultaneously or succes sively. In the case of signal transduction, for example, a ligand first binds to the cell sur face receptors, the ligand-receptor complex is internalized, signals are transferred one by one through the intracellular signal molecules, and finally the signal reaches the target molecules. In these processes, the signal is mediated mainly by phosphorylation, and this phosphorylation is transduced from upstream to downstream by transient in teractions of the donors with the acceptors. Phosphorylation and de-phosphorylation themselves are also characterized as transient interactions between phosphates and tar get proteins. 272 pp. Englisch. Codice articolo 9784431669722
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Real-Time Analysis of Biomolecular Interactions: Applications of BIACORE
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Real-Time Analysis of Biomolecular Interactions
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Springer Japan, Springer Japan Nov 2012, 2012
ISBN 10: 4431669728
ISBN 13: 9784431669722
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Da: buchversandmimpf2000, Emtmannsberg, BAYE, Germania
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Taschenbuch. Condizione: Neu. Neuware -Elaborate and complex interactions among the molecules that make up all living or ganisms are the basis by which those organisms carry out their existence. All metabo lism or vital activities of living organisms are attributed to the interactions of numbers of biomolecules, including protein-protein interactions. Cell growth and differentia tion, development and generation or regeneration of tissue, and the occurrence of or protection against various diseases are triggered, conducted, and maintained by the complex sequential interactions among molecules both inside and outside the cells. Each protein has its own function, but it cannot function by itself. For example, enzymes catalyze reactions only through transient interaction with their substrates. In some cases, proteins carry out their functions only after they form a multi subunit com plex. GenerallY, proteins, nucleotides, lipids, sugars, and other small molecules func tion through the interaction of mUltiple molecules either simultaneously or succes sively. In the case of signal transduction, for example, a ligand first binds to the cell sur face receptors, the ligand-receptor complex is internalized, signals are transferred one by one through the intracellular signal molecules, and finally the signal reaches the target molecules. In these processes, the signal is mediated mainly by phosphorylation, and this phosphorylation is transduced from upstream to downstream by transient in teractions of the donors with the acceptors. Phosphorylation and de-phosphorylation themselves are also characterized as transient interactions between phosphates and tar get proteins.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 272 pp. Englisch. Codice articolo 9784431669722
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Real-Time Analysis of Biomolecular Interactions : Applications of BIACORE
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Springer Japan, Springer Japan, 2012
ISBN 10: 4431669728
ISBN 13: 9784431669722
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Da: AHA-BUCH GmbH, Einbeck, Germania
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Taschenbuch. Condizione: Neu. Druck auf Anfrage Neuware - Printed after ordering - Elaborate and complex interactions among the molecules that make up all living or ganisms are the basis by which those organisms carry out their existence. All metabo lism or vital activities of living organisms are attributed to the interactions of numbers of biomolecules, including protein-protein interactions. Cell growth and differentia tion, development and generation or regeneration of tissue, and the occurrence of or protection against various diseases are triggered, conducted, and maintained by the complex sequential interactions among molecules both inside and outside the cells. Each protein has its own function, but it cannot function by itself. For example, enzymes catalyze reactions only through transient interaction with their substrates. In some cases, proteins carry out their functions only after they form a multi subunit com plex. GenerallY, proteins, nucleotides, lipids, sugars, and other small molecules func tion through the interaction of mUltiple molecules either simultaneously or succes sively. In the case of signal transduction, for example, a ligand first binds to the cell sur face receptors, the ligand-receptor complex is internalized, signals are transferred one by one through the intracellular signal molecules, and finally the signal reaches the target molecules. In these processes, the signal is mediated mainly by phosphorylation, and this phosphorylation is transduced from upstream to downstream by transient in teractions of the donors with the acceptors. Phosphorylation and de-phosphorylation themselves are also characterized as transient interactions between phosphates and tar get proteins. Codice articolo 9784431669722
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Real-Time Analysis of Biomolecular Interactions: Applications of BIACORE
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Real-Time Analysis of Biomolecular Interactions: Applications of BIACORE
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Paperback. Condizione: Brand New. reprint edition. 267 pages. 9.26x6.11x0.62 inches. In Stock. Codice articolo x-4431669728
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Real-Time Analysis of Biomolecular Interactions: Applications of BIACORE
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Real-Time Analysis of Biomolecular Interactions: Applications of BIACORE
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Paperback. Condizione: Like New. Like New. book. Codice articolo ERICA80044316697286
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