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The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF’ cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low.
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Editore:
LAP LAMBERT Academic Publishing Feb 2020, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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Da: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germania
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Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low. 64 pp. Englisch. Codice articolo 9786200587886
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Editore:
LAP LAMBERT Academic Publishing, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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Taschenbuch
Da: AHA-BUCH GmbH, Einbeck, Germania
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Taschenbuch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low. Codice articolo 9786200587886
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Editore:
LAP LAMBERT Academic Publishing, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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Da: moluna, Greven, Germania
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Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Shrivastava NeerajDr Neeraj Shrivastava (Ph.D) is working as Assistant Professor in the Department of Veterinary Microbiology at N.D.V.S.U.,Jabalpur(M.P.)The present work is his MVSc thesis.Dr Shrivastava is a gold medalist in B.V.Sc. Codice articolo 385895895
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Editore:
LAP LAMBERT Academic Publishing Feb 2020, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
Nuovo
Taschenbuch
Da: buchversandmimpf2000, Emtmannsberg, BAYE, Germania
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Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 64 pp. Englisch. Codice articolo 9786200587886
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Editore:
LAP LAMBERT Academic Publishing, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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Da: preigu, Osnabrück, Germania
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Taschenbuch. Condizione: Neu. Expression and Characterization of Nucleocapsid (N) Gene of R.[.] | Neeraj Shrivastava (u. a.) | Taschenbuch | 64 S. | Englisch | 2020 | LAP LAMBERT Academic Publishing | EAN 9786200587886 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Codice articolo 118114926
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Editore:
LAP LAMBERT Academic Publishing, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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Da: Mispah books, Redhill, SURRE, Regno Unito
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paperback. Condizione: New. NEW. SHIPS FROM MULTIPLE LOCATIONS. book. Codice articolo ERICA82962005878846
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