Systematic Analysis of Working Models to Detect Thioredoxin Activities: Detecting Thioredoxin Activity by Systemic Analytical Tools - Brossura

Verma, Neha; Abbot, Vikrant; Sharma, Mrinal

 
9786206844068: Systematic Analysis of Working Models to Detect Thioredoxin Activities: Detecting Thioredoxin Activity by Systemic Analytical Tools
Brossura
ISBN 10: 6206844064 ISBN 13: 9786206844068
Casa editrice: LAP LAMBERT Academic Publishing, 2023

Sinossi

Thioredoxin (Trx) (12 kDa M.W.) is a ubiquitous antioxidant protein which controls the oxidative stress and redox signaling with thioredoxin reductase (TrxR) and NADPH. Trx acts as an electron donor for ribonucleotide reductase (RNR), methionine sulfoxide reductase (Msr), and Trx peroxidase (peroxiredoxin). Trx plays an important role in DNA synthesis, reduction of the disulfide bond, recycling of peroxiredoxin (Prx), and as an electron donor. Various in vivo and in vitro assays are used to check the activity of Trx. This work is focused on the study of in vivo and in vitro methods for Trx activity which have been used in the different model system. Different methods were studied to evaluate the activity of Trx in different samples. Insulin reduction assay is more commonly used in vitro method because insulin acts as effective substrate for activity of Trx. Fluorescence, DTNB (5, 5ʹ-dithiobis (2-nitrobenzoic acid)) reduction, spectrophotometric or micro-method insulin reduction, and disulfide cross-linking interaction in vitro assays while Luciferase, cloning, and wort in vivo assays are used for the activity of the Trx system.

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Editore
LAP LAMBERT Academic Publishing
Data di pubblicazione
2023
Lingua
Inglese
ISBN 10 
6206844064
ISBN 13 
9786206844068
Rilegatura
Copertina flessibile
Numero di pagine
52
Contatto del produttore
non disponibile
Persona responsabile
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