Development of a Fully Integrated Sample-in-answer-out System for Automatic Genetic Analysis - Rilegato

Zhuang, Bin

Rilegato

ISBN 10: 9811047529 ISBN 13: 9789811047527

Casa editrice: Springer Nature, 2017

Riassunto

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.

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Informazioni sull'autore

For internal review:

Dr. Bin Zhuang

Supervisor: Prof. Jing Cheng, School of Medicine, Tsinghua University

Current Affiliation: National Engineering Research Center for Beijing Biochip Technology, Capitalbio Corporation

Research Area: Biomedical engineering, microfluidic, nucleotide analysis, electrophoresis, biochemistry

Publications:

[1] Gan, W.P.,# Zhuang, B.,# Zhang, P.F., Han, J.P., Li, C.X., and Liu, P. (2014). A filter paper-based microdevice for low-cost, rapid, and automated DNA extraction and amplification from diverse sample types. Lab on a Chip 14, 3719-3728.

[2] Zhuang, B., #  Gan, W.P., Wang, #  S.Q., Han, J.P., Xiang, G.X., Li, C.X., Sun, J., Liu, P. (2015). A fully automated sample preparation microsystem for genetic testing of hereditary hearing loss using two-color multiplex allele-specific PCR. Analytical Chemistry, 87(2):1202-1209.

[3] Zhuang, B., #  Han, J.P., #  Xiang, G.X., Gan, W.P., Wang, S.Q., Wang, D., Wang, L., Sun, J., Li, C.X., Liu, P. (2016). A fully integrated and automated microsystem for rapid pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. Lab on a Chip 16, 86-95.

Dalla quarta di copertina

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an “in-situ” PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system’s performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid “sample-in-answer-out” testing in routine clinical practice.

Dettagli dell'edizione

• Editore: Springer Nature
• Data di pubblicazione: 2017
• Lingua: Inglese
• ISBN 10: 9811047529
• ISBN 13: 9789811047527
• Rilegatura: Copertina rigida
• Numero edizione: 1
• Numero di pagine: 140
• Contatto del produttore: non disponibile
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