Reverse Phase Protein Arrays: From Technical and Analytical Fundamentals to Applications: 1188 - Brossura

Brossura

ISBN 10: 9813297573 ISBN 13: 9789813297579

Casa editrice: Springer Nature, 2021

Riassunto

This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.

Su questo libro

Informazioni sull?autore

Tesshi Yamada, M.D., Ph.D. 

Professor of International University of Health and Welfare, Narita, Japan

Satoshi Nishizuka, M.D., Ph.D. 

Professor of Iwate Medical University, Japan

Gordon Mills, M.D., Ph.D. 

Professor of University of Texas, MD Anderson Cancer Center

Lance Liotta, M.D., Ph.D. 

Co-Director of the Center for Applied Proteomics and Molecular Medicine, George Mason University

Dalla quarta di copertina

This book provides practical guidance on all aspects of reverse phase protein array (RPPA) technology, which permits the quantification of protein levels in cell or tissue lysates. In addition, the latest results are presented from laboratories across the world where experts are successfully running the challenging RPPA data platform. After an introductory chapter by the inventor of the RPPA technique, the demanding task of lysate preparation is explained. Subsequent chapters review printing platforms and analytical platforms and discuss antibody screening in detail. The role of RPPA data in integrative analyses is thoroughly examined, and the various applications of RPPA, for example to identify molecular targeting drugs or evaluate cancer drug efficacy, are discussed with reference to the latest knowledge. It is hoped that the book will foster the implementation of RPPA by documenting the practical details of the technology and by revealing its great potential. It will be of high value for researchers who plan to build an RPPA platform in their institute and for researchers, clinicians, and companies who use RPPA through collaboration with an existing RPPA facility.

Dettagli dell'edizione

• Editore: Springer Nature
• Data di pubblicazione: 2021
• Lingua: Inglese
• ISBN 10: 9813297573
• ISBN 13: 9789813297579
• Rilegatura: Copertina flessibile
• Numero edizione: 1
• Numero di pagine: 276
• Redattore: Yamada Tesshi, Nishizuka Satoshi S., Mills Gordon B., Liotta Lance A.
• Contatto del produttore: non disponibile
• Persona responsabile: CMN Printpool Inh. Mathis Neumann; https://www.also.com/ec/cms5/en_6000/6000/index.jsp; ; Friedrich-Karl-Str. 9; Hamburg; Germania