Da: GreatBookPrices, Columbia, MD, U.S.A.
Condizione: New. Codice articolo 50026046-n
Quantità: Più di 20 disponibili
Da: California Books, Miami, FL, U.S.A.
Condizione: New. Codice articolo I-9798230131236
Quantità: Più di 20 disponibili
Da: PBShop.store US, Wood Dale, IL, U.S.A.
PAP. Condizione: New. New Book. Shipped from UK. Established seller since 2000. Codice articolo L2-9798230131236
Quantità: Più di 20 disponibili
Da: GreatBookPrices, Columbia, MD, U.S.A.
Condizione: As New. Unread book in perfect condition. Codice articolo 50026046
Quantità: Più di 20 disponibili
Da: Ria Christie Collections, Uxbridge, Regno Unito
Condizione: New. In English. Codice articolo ria9798230131236_new
Quantità: Più di 20 disponibili
Da: GreatBookPricesUK, Woodford Green, Regno Unito
Condizione: New. Codice articolo 50026046-n
Quantità: Più di 20 disponibili
Da: GreatBookPricesUK, Woodford Green, Regno Unito
Condizione: As New. Unread book in perfect condition. Codice articolo 50026046
Quantità: Più di 20 disponibili
Da: AHA-BUCH GmbH, Einbeck, Germania
Taschenbuch. Condizione: Neu. Neuware - The CRISPR/Cas9 system is a naturally occurring, adaptive heritable immune system which defends invading phage attack and present in the whooping numbers of prokaryotes.This has been repurposed as a powerful tool which is guided by RNA and targets DNA for genome engineering which works by making site-specific DNA break and its subsequent repair by cellular machinery. The simplicity and multiplexity of the CRISPR system make it more sought after than other older counterparts viz. ZFNs and TALENs, and this has led to harnessing of CRISPR in gene therapy programmable gene regulation, cellular imagings, synthetic biology and nucleic acid detection among others. The CRISPR genome editing system includes an endonuclease, Cas9 derived from type II CRISPR-Cas system and a chimeric single-guide RNA (sgRNA). Cas9 and sgRNA forms ribonucleoprotein (RNP) binary complex and upon recognition of a protospacer adjacent motif (PAM) it binds to the substrate DNA, and forms ternary complex by unwinding the PAM upstream region with subsequent formation of R-loop. Successful R-loop formation displaces PAM- containing DNA strand and results in formation of a heteroduplex only when the target DNA strand finds complementary to sgRNA sequence. It is worth mentioning Cas9 remains inactive inthe absence of sgRNA and sgRNA binding induces structural changes in Cas9 from apo toactive form. Codice articolo 9798230131236
Quantità: 2 disponibili