Advanced Technologies for Protein Complex Production and Characterization

Lingua: inglese

Editore: Springer International Publishing AG, CH, 2016

3319272144 / 9783319272146

Serie: Libro 232 di 544 - Advances in Experimental Medicine and Biology

Da: Rarewaves.com UK, London, Regno UnitoRarewaves.com UK

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This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists.Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance.This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.…

Codice articolo LU-9783319272146

Titolo
Advanced Technologies for Protein Complex Production and Characterization
Autore
M. Cristina Vega
Editore
Springer International Publishing AG, CH
Anno di pubblicazione
2016
Condizione
New
Rilegatura
Hardback
Lingua
inglese
ISBN 10
3319272144
ISBN 13
9783319272146
Edizione
2016 ed.
Serie
Libro 232 di 544: Advances in Experimental Medicine and Biology

Rarewaves.com UK

London, Regno Unito

Venditore con 5 stelle

Venditore AbeBooks dal 11 giugno 2025

Tariffe di spedizione da Regno Unito a U.S.A.

ArticoloDa 60 a 60 giorni lavorativiDa 60 a 60 giorni lavorativi
Primo articoloEUR 77,65EUR 119,46
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