<p>The 18<sup>th</sup> ESACT meeting was celebrated in Granada (Spain) in May 2003, and was entitled "Animal Cell Technology Meets Genomics", in order to reflect that the emerging technologies in the area of genomics, proteomics and other "-omics"-type disciplines will provide key technological assets to increase knowledge and open new horizons in animal cell technology. During the meeting a variety of top-class emerging technologies were presented together with the lastest advances in more mature industrial areas. The meeting was opened by a first session devoted to the understanding of basic cellular mechanisms, and four sessions focused on applied aspects of animal cell technology: Cell-based therapies and gene-based therapies, target discovery and biopharmaceuticals. The Granada Meeting has also seen a special focus on forefront industrial case studies. The spirit and scientific excellence of the 18<sup>th</sup> ESACT meeting is now reflected in different chapters of the book. The book presents, in form of short papers, a high number of the contributions to the meeting, and has been prepared with the aim to provide a relevant reference of the current research efforts in Animal Cell Technology.</p>
Meeting Committees; ESACT Executive Committee; Sponsors; Companies Participating in the Trade Exhibition; Introduction: Animal Cell Technology Meets Genomics; Acknowledgements; List of Participants; CHAPTER 1: CELLULAR MECHANISMS; Effect of osmotic pressure on GS-NSO expression system, Wu M., et at.; Molecular approaches to influence epigenetic effectors of transient and stable transgene expression in mammalian cells, Hacker D. et al.; Genomic exploration on Chinese hamster ovary cells, de Leon Gatti M. et al.; Roles for glycosylation in receptor-ligand interactions in the immune system, Rudd, P.M. et al.; Investigations on mannose-6-phosphate receptor mediated protein uptake, Duerrschmid M., Jursik, et al.; Analysis of multiple apoptosis parameters using a microfluidic chip-based system, Valer M. et al.; Inhibiting apoptosis in cell culture using multiple inhibitors, Figueroa, B. et al.; Pausing of mammalian cells by cold exposure, limits and Opportunities, Hunt L. et al.; The functional competence of animal cells: analysis of the secretory pathway, Racher A.J. et al.; Proteome analysis of recombinant CHO cells under hyperosmotic stress, Lee M.S. et al.; Expression profiling analysis of sodium butyrate-induced Chinese hamster ovary cells in defined medium, Melville M.W. et al.; Intracellular nucleotide pools for optimizing productoriented transient transfection of HEK293 cells in suspension, Bassani Molinas M.M. et al.; Impact of yeast pyruvate carboxylase on the productivity of animal host cell lines, Bollati Fogolín M. et al.; Environmental effects of lactate on High-Five™ insect cell Metabolism, Drugmand J.-C. et al.; Correlation of intracellular nucleotide pools to amino acid concentrations in culture media by the application of multivariate methods, Hesse F. et al.; Engineering Trichoplusia ni (High Five™) insect cells to express a cytosolic pyruvate carboxlase enzyme improves the viability of the cells in batch, fed-batch and perfusion cultures, Locas M.-C. et al.; Insect cell culture medium selection and optimisation based on monitoring and economical considerations, Lecina M. et al.; Antiapoptotic activity of synthetic and natural peptides, Franek F. et al.; Effect of antiapoptotic genes expression on cell growth and monoclonal antibody productivity in a hybridoma cell line, Juanola S. et al.; Amplified dicistronic expression units mediate apoptosis protection in CHO-DG44 cells adapted for growth in serum-free media, impact on mitochondria copy number, Meents H. et al.; Effect of reduced water on the apoptotic cell death triggered by oxidative stress in pancreatic ß HIT-T15 cell, Shirahata S. et al.; Characterization of apoptosis in a CHO cell line cultivated in batch and continuous culture: Effect of medium, specific perfusion rate and chemical inhibitors, Thrift J. et al.; Hydrogen peroxide-induced cellular senescence is regulated via two different pathways, Yoshizaki K. et al.; Identification of autocrine factors influencing proliferation in serum free cultures of Trichoplusia ni cells, Eriksson U. et al.; The effects of insulin and Long™R3IGF-I on Chinese hamster ovary cells: cell survival, receptor activation and second messenger pathways. Yandell C.A. et al.; Conditioned medium factors in protein-free cultures of NS0 cells, Spens E. et al.; Production of human anti-peptide antibody for clinical use by in vitro immunization, Tamura, T. et al.; Optimization of in vitro immunization protocol to produce antigen specific human monoclonal antibody by demonstrating the role of IL-10, Xu Q.H. et al.; Extracellular histone H4 from SF9 cells is antimicrobial, Calles K. et al.; Analyzing calcium phosphate transfection of adherent CHO cells using microscopic live imaging, Grosjean F. et al.; Effects of multiplicity of infection (MOI) and cell cycle on Baculovirus infection kinetics, Haas R. et al.; Non-freezing preservation and subsequent recovery of hepatocytes under pO2- and pH-controlled conditions for continuous supply of cells for bioartificial liver support, Iding K. et al.; Selection of MDCK subclones with various genotypic and phenotypic markers: sensitivity to influenza virus infection, Kessler N. et al.; Extracellular metalloproteases and their inhibitors expressed by the IFN-ã producing CHO-320 cell line, Mols J. et al.; Novel small-molecule AKT1 inhibitors discovered by Redistribution™-based high-throughput screening. Præstegaard M. et al.; Influence of mistletoe extracts and its components on invitro physiology of cancer cells, Sidler F. et al.; Rapid in-process monitoring of antibody integrity, purity and titre-2100 Bioanalyser (Lab on a chip) study, Nesredin A. et al.; Toxicological evaluation of haine river waste-water samples to appropriately sensitised cultured Fathead Minnow cells compared with the microtox® method, Dierickx P.J. et al.; CHAPTER 2: CELL BASED THERAPIES; 3D-cultivation and characterisation of osteogenic cells for the production of highly viable bone tissue implants, Barthold M. et al.; Carbon source has a considerable influence on extracellular matrix formation, demonstrated on specific collagen expression of primary human tenocytes, Möbest D. et al.; Design and characterization of cardiomyocyte-derived micro issues, Kelm M.J. et al.; Process development for standardized generation of monocyte-derived dendritic cells: applicability in breast cancer immunotherapy, Bohnenkamp H.R. et al.; Stemline™ hematopoietic stem cell expansion medium, a serum-free medium for expansion of CD34+ hematopoietic stem cells and progenitors, Leugers S. et al.; Mesenchymal stem cells: a very first step towards large scale cell culture strategies transplantation, Bensellam M. et al.; Expansion of murine embryonic stem cells on microcarriers-functional characteristics and scale-up potential, Burg M. et al.; In vitro cultivation of rabbit mesenchymal stromal cells on 3D bioresorbable calcium phosphate scaffolds for the generation of bone tissue implants, Fargali S. et al.; Establishment and characterization of human medullary thyroid carcinoma cell lines for immunotherapy, Stradler G. et al.; Suppression of cell growth by platinum nanocolloids as scavengers against reactive oxygen species, Hamasaki T. et al.; Comparison of fluidized bed and fed batch reactor cultures for production of anti-HIV-antibody, Hinterleitner P. et al.; Suppression of oxidative stress-induced apoptosis of neuronal cells by electrolyzed-reduced water, Kashiwagi T. et al.; Spheroid formation by encapsulation of cancer cells to mimic small size tumors, Marc A. et al.; Bioreactor for continuous biomechanical characterization of cellular systems and tissue-engineered biohybrid tissues, Möbest D. et al.; Membrane-separated cocultivation of cord blood hematopoietic stem cells with stromal cell lines, Fischbach T. et al.; Anti-apoptotic genes protect an insulinoma cell line from cell death induced by hyperglycemia, Ogawa A. et al.; Preparation and characterization of an idiotype-dendritic cell vaccine for immunotherapy of multiple myeloma, Opalka B. et al.; Pretreatment with polycations enhances Adenoviral infection levels in serum-free PER.C6™ cells as well as HEK 293 cells, Padilla J. et al.; Optimizing the production of cardiomyocytes from mouse embryonic stem cells in a 2L stirred tank reactor, Schroeder M. et al.; Establishment and characterization of CD4+ Killer-T-Cells (KTCs), Voglauer R. et al.; CHAPTER 3: GENE BASED THERAPEUTICS; Successful development of a robust adenovirus production process primarily relies on a better understanding of packaging cell line physiology and vector replication kinetics, Kamen A. et al.; Retroviral vector stability: inactivation kinetics and membrane properties, Cruz P. et al.; Comparison of host cell lines and production methods for a new generation of oncolytic adenoviral vectors, Forestell S. et al.; Process development for a veterinary vaccine against heartwater, Marcelino I. et al.; A new method for the quantitative determination of enveloped viral particles, Beer C. et al.; Development of a 293T cell line for the production of virus like particles in bioreactors, Costa M.J.L. et al.; Effect of MOI and medium composition on adenovirus infection kinetics, Ferreira T. et al.; ION-exchange chromatography based strategies for purification of viral vectors, Vasi J. et al.; Scaling up of endothelial cells culture for the production of a rickettsiae vaccine: evaluation of the importance of the interference effects, Hendrick V. et al.; CHAPTER 4: TARGET DISCOVERY; Inducible gene expression for antibiotic drug discovery and diagnostics Weber W. et al.; Nucleic acid capture assay: a novel high-throughput method for direct mRNA quantitation Van de Goor J. et al.; High throughput transient transfections of HEK293EBNA cells: development of an integrated expression purification process at 100 ml scale, Heine H., Rey L. et al.; Transient gene expression in suspension HEK293 cells: application to large-scale protein production, Baldi L., Jacquet R. et al.; Monoclonal antibody production: determination of appropriate system and medium for production enhancement and decrease of overall production time, Fritchman K. et al.; The secrets of transfection in serum-free suspension culture Geisse S. et al.; From genes to proteins: full open reading frame (ORF) clones and their application in functional research, Goedde A. et al.; A versatile disposable culture system for high throughput screening of process parameters and production cell lines, Jordan M. et al.; Long time storage of pre-inoculated multi-well plates for cell-based assay kits used for drug identification and characterization, Loa A. et al.; Comparative secretion levels of recombinant proteins between insect cell-baculovirus and mammalian HEK293EBNA cells, Losberger C. et al.; Microfluidic technology applied to protein sizing and Quantitation, Valer M. et al.; CryoStock – a software for cell culture management, Zerlin M. et al.; CHAPTER 5: BIOPHARMACEUTICALS; Targeting the human Immunoglobulin loci for high level heterologous gene expression, Winkler K. et al.; MAR elements as tools to increase protein production by CHO cells, Girod, P.-A. et al.; Cell line engineering for production of therapeutic antibody glycoforms with increased biological activity, Umana P. et al.; Development of a metabolically optimized fermentation process based on glucose-limited CHO perfusion culture; Applications of quasi real-time metabolic flux analysis in mammalian cell culture process, Goudar C. et al.; Data-based modelling of cell cultures, Cunha-Bakeev C. et al.; Cell culture and downstream processing integration using ProteinChip® technology. Application to protein analysis and purification in biopharmaceutical manufacturing, Bengio S. et al.; The prospects for insect cells for use as a cell substrate for the production of biotherapeutics, Galbraith D.N.; High-performance cell culture platform technology for Mab production, Chang D.Y.H. et al.; Development and integration of a new animalcomponent-free process for the production of UK- 279,276, Pluschkell S. et al.; Towards an affinity-based capture system for the isolation of high expression cells using a co-expressed surface protein, Derouazi M. et al.; Effect of doxycycline-regulated calnexin and calreticulin expression on specific thrombopoietin productivity of recombinant CHO cells, Chung J.Y. et al.; Recombinant glycoprotein expression. Development of a homologous CHO expression system, Noone C. et al.; Generation of recombinant antibody manufacturing cell lines using cell-cell fusion, Zeng W. et al.; Cloning and expression of biopharmaceuticals in CHO.K1 and BHK-21 cells, Castilho L.R. et al.; Camel single-domain antibodies as modular building blocks to make bivalent constructs for use in immunotheraphy of cancer, Cortez-Retamozo V. et al.; Establishment of efficient cloning method for variable region genes of antigen specific human monoclonal antibody, Matsumoto S. et al.; From gene to monoclonal antibody: efficient screening by cell sorting, Borth N. et al.; Development of an integrated strategy for recombinant cell line selection, Castillo A.J. et al.; Gene transfer to animal cells in culture by microinjection: A novel tool to create recombinant cell lines? Derouazi M. et al.; Evaluation of the stability of NS0 cell lines expressing recombinant human IgG, Forrest-Owen W. et al.; High throughput screening and selection of single cells in suspension using fluorescence parameters, Giese C. et al.; Control of key parameters in the development of mammalian production clones Kunert R. et al.; Establishment of screening systems for recombinant cell lines: early prediction of the suitability as a production clone, Lattenmayer C. et al.; Characterisation of CHO subclones showing profound changes in performance when propagated in different cultivation systems, Trummer E. et al.; High level production of recombinant IgG in the human cell line PER.C6™, Yallop C. et al.; Creating a new medium to help meet the variable nutritional requirements of Chinese hamster ovary (CHO) cell clones, Deeds Z.W. et al.; Development of an efficient medium optimization kit for factorial matrix design - a statistical approach to increase cell growth and productivity of recombinant CHO cells, Delong B. et al.; An efficient approach to cell culture medium optimization – a statistical method to medium mixing, Gifford J. et al.; Virus inactivation in foetal calf serum by a combined, treatment of gamma-irradiation and UV-C irradiation, Hermann H. et al.; Advances in media optimization: two automated approaches that increase expression while reducing development time, Holdread S. et al.; The importance of cholesterol for insect cell growth and baculovirus production, Lua L.H.L. et al.; Use of rapeseed proteins and peptides as supplements in insect cell cultures, Marc A. et al.; Nutritional performance of wheat gluten -derived protein hydrolysates in hybridoma cell cultures, Martens D.E. et al.; SyntheChol™ synthetic cholesterol for cholesterol dependent cell culture. Development of non-animal derived chemically defined NS0 medium, Talley D. et al.; NS0 derivatives: MAb production in large-scale SFM formats, Manwaring J. et al.; Silk protein sericin accelerates proliferation of various mammalian cells, Terada S. et al.; Image analysis based realtime-control of glucose concentration, Brueckerhoff T. et al.; On line analysis of microcarrier cultivations, Burzlaff A. et al.; Error analysis during estimation of metabolic fluxes through metabolite balancing, Goudar C. et al.; Generalized logistic equation modeling of mammalian cell batch cultures, Goudar C. et al.; In-situ microscopy based monitoring of mammalian cell culture processes, Joeris K. et al.; A novel disposable microtube for rapid assessment of biomass in cell cultures, Jordan M.; Intracellular monitoring of baculovirus infection of Sf9 insect cells and r-protein production using anti-alpha-1,3/4 fucosyltransferase antibodies and flow cytometry, Marc A. et al.; Effect of glucose and glutamine concentration on metabolism of animal cells in chemostat culture, Matsuoka H. et al.; Evaluation of a novel capacitance probe for on-line monitoring of viable cell densities in batch and fedbatch animal cell culture processes, Schmid G. et al.; Sodium butyrate enhancement of protein expression in HeLa cells: follow-up of a chimeric reporter gene behaviour for process development, Bassens C. et al.; Scalable, serum-free, high efficiency transfection of CHO Cells using an economical DNA transfer vehicle, Girard P. et al.; Process optimization for an NS0-derived hybridoma cell line in a chemically defined, protein-free medium, Johnson J.M. et al.; Impact of change in fermentation process pH and dissolved carbon dioxide concentration on secreted antibody structure and cell physiology of a GS-NS0 cell line expressing a human antibody,...
